The microstructure of the nucleus, one of the most studied but least understood cellular organelles, is the subject of much debate. Through the use of particle nanotracking, we detect and quantify the micro-organization as well as the viscoelastic properties of the intranuclear region in single, live, interphase somatic cells. We find that the intranuclear region is much stiffer than the cytopl...

BACKGROUND Neuronal intranuclear inclusion disease (NIID) is a progressive neurodegenerative disease characterized by eosinophilic hyaline intranuclear inclusions in neuronal and somatic cells. Because of the variety of clinical manifestations, antemortem diagnosis of NIID is difficult. METHODS Seven skin biopsy samples from patients with familial NIID were evaluated histochemically, and the ...

Nucleic acids and regulatory proteins involved in gene expression and DNA replication are localized in specialized subnuclear domains that can be visualized by microscopy. However, the rules that govern the compartmentalization of the nucleus into these discrete domains have been elusive. From an historical perspective, the concept of nuclear subdivisions as functional compartments initially fo...

The aim of this study was to test the efficacy of eosinophilic count as a broad indicator in prognosticating mortality in surgical intensive care unit (ICU). 437 patients admitted to the emergency ward were evaluated for eosinophilc count and absolute eosinophilic count. In a ten years study undertaken from 2004 to 2014, patients admitted to the emergency ward were evaluated for eosinophilic co...

Neuronal intranuclear inclusions have been found in the brain of a transgenic mouse model of Huntington's disease and in necropsy brain tissue of patients with Huntington's disease. We suggest that neuronal intranuclear inclusions are the common neuropathology for all inherited diseases caused by expansion of polyglutamine repeats. We also suggest that patients with a pathological diagnosis of ...

Background: Eosinophilic gastroenteritis is an increasingly recognised condition with variable presentations. Eosinophilc involvement of the biliary tract and the pancreas is extremely rare and usually occurs in the setting of idiopathic hypereosinophilic syndrome. Case report: We report a case of 12 years old male child who was receiving steroids after a diagnosis of eosinophilic gastroenterit...

Recent data indicate that the chromatoid body typical of rat spermatogenesis may contain RNA synthesized in early spermatids by the haploid genome. Analyses of living step-1 and step-3 spermatids by time-lapse cinephotomicrography have shown that the chromatoid body moves in relation to the nuclear envelope in two different ways. Predominantly in step 1, the chromatoid body moves along the nucl...

Morphological investagations were carried out on basophilic intranuclear and cytoplasmic inclusion bodies in epithelial cells of the hair bulbs of anagen hair follicles in wild foxes affected with an abnormal hair coat condition. The inclusions , which were DNA-positive, contained numerous minute virus-like particles ("FMVP"). The particles, having a diameter of approximately 13 nm, had an arra...

Mutations in the valosin-containing protein (VCP) gene are known to cause various neurodegenerative disorders. Here, we report 8 Japanese patients [6 men, 2 women; median age at onset: 49.5 (range, 35–58) years] from 5 unrelated families with VCP missense mutations. Although 7 of were diagnosed either inclusion body myopathy or amyotrophic lateral sclerosis, 1 patient showed demyelinating polyn...