An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected gilt-head seabream (Sparus aurata, L.) has been developed. A specific antiserum against a 60 kDa viral protein has been proven to be an appropriate tool for LCDV diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. The sensitivity of this technique vari...

The main source of calcium carbonate (CaCO₃) in the ocean comes from the shells of calcifying planktonic organisms, but substantial amounts of CaCO₃ are also produced in fish intestines. The precipitation of CaCO₃ assists fish in intestinal water absorption and aids in whole body Ca²⁺ homeostasis. Here we report that the product formed in the intestinal lumen of the gilt-head seabream, Sparus a...

The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20 degree...

This study determined the effect of prey DHA on larval gilthead sea bream (GSB; Sparus aurata ) photoreceptor abundance, rhodopsin expression, and growth performance. It was carried out in a twenty-eight 400 l conical tank system that stocked with 100 viable GSB eggs/l/tank. allowed testing 4 levels rotifer DHA; 0.99 (Low; L), 1.9 (Intermediate low; I-L), 3.2 high; I-H) 12.1(High; H) mg DHA/g D...

a r t i c l e i n f o Keywords: Sparus aurata Video-tracking Swimming activity Chronotoxicity MS-222 Anaesthesia Fish are frequently exposed to anaesthetics since their use is necessary in several aquaculture procedures. The aim of this study was to investigate the existence of day–night differences in the toxicity and effectiveness of a common fish anaesthetic (MS-222) in juvenile gilthead sea...

Estuarine and/or migratory teleosts may experience large and rapid changes in external [Ca(2+)]. Previous studies have largely centred on the physiological mechanisms that maintain a constant plasma [Ca(2+)] in the face of such external fluctuations, but little work has been directed to examining how these changes may originally be detected. We present evidence that the olfactory system of the ...

the genus trachylepis fitzinger, 1843 encompasses two-three species in iran: trachylepis vittata (olivier, 1804) distributed in western iran, west of the zagros mountains; t.septemtaeniata (reuss, 1834) in southern regions of the zagros mountains and t. aurata transcaucasica chernov, 1926 from northern to central parts of the zagros mountains. for study of geographic variation in the latter two...