نتایج جستجو برای: lexsy expression system
تعداد نتایج: 2986708 فیلتر نتایج به سال:
Background and Objective: Human embryonic stem cells (hESCs) can differentiate in vitro into spontaneously contracting cardiomyocytes and produce a model to investigate the early developmental stages of this system. After removing of cells from their feeder layer, hESCs create embryoid bodies (EB). Plating of EB results in developing areas of beating cells. In the present study, cardiomyocyte g...
there are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. bicistronic system has disadvantages e.g. compared with two-promoter system. in this paper, a simple method based on spliced overlap extension by polymerase chain reaction (soe-pcr) technique was demonstrat...
The throughput enhancement of Space-Time Spreading (STS)-based Multicarrier Direct Sequence Code Division Multiple Access (MC DS-CDMA) system is investigated in this paper. Variable Spreading Factor (VSF) is utilized to improve the data throughput of the system. In this contribution, an analytical approach is proposed to compute a new expression for the Bit Error Rate (BER) performance of t...
Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...
In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...
بمنظور پی بردن به چگونگی مناسبات احتمالی میان خصوصیات شخصیتی با موفقیت تحصیلی و گزینش دانشکده، فرم فارسی آزمون شخصیتی لنیون به 178 نفر دانشجوی پسر و دختر سال دوم دانشکده های گوناگون دانشگاه شیراز و 346 نفر دانش آموز پسر و دختر کلاسهای ششم مدارس نواحی مختلف شهر شیراز داده شد. نتایج حاصله نشان داد که : 1 - در مورد پسرهای دانشجو همبستگی مثبت معنی داری بین عامل شخصیتی alienation و موفقیت تحصیلی وجو...
Background : The aim of the present study was to identify a suitable cell line for studies of recombinant protein production in eukaryotic system. After transfection, altered expression levels of RNA and its target protein were analyzed. Materials and Methods: To investigate the in vitro expression of E6 protein of human papillomavirus type 16 in cell culture, the plasmid pcDNA3-E6, and two di...
objectives the cause of rheumatoid arthritis (ra) as a chronic inflammatory autoimmune disease is still unknown. it appears that both genetic and environmental factors play a role in its pathogenesis. recent studies reveal that in addition to the cns, immune cells synthesis neurotransmitters so that these catecholamines can regulate immune functions. the aim of this study is to evaluate the dop...
background human preimplantation embryonic cells share many similarities with cancer cells such as ability to self-renew, unlimited proliferation and maintenance of the undifferentiated state. embryo-cancer sequence a (ecsa), also known as developmental pluripotency associated-2 (dppa2), is a cancer testis antigen (cta) with unclear biological function yet. objective: ctas are expressed normall...
Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...
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