Dissolved Fe(II) in seawater is deemed an important micronutrient for microbial organisms, but its analysis is challenging due to its transient nature. We conducted a series of Fe(II) method comparison experiments, where spikes of 5 to 31 nM Fe(II) were added to manipulated seawaters with varying dissolved oxygen (37 to 156μM) concentrations. The observed Fe(II) concentrations from four analyti...

The two most common ways to induce chemiluminescence in purely organic, nonbiological systems are to treat either oxalate esters or luminol derivatives with basic hydrogen peroxide. Both these types of mixtures emit light at relatively short wavelengths, which are not ideal for applications in biotechnology. Luminol, for instance, emits in the range 420–450 nm, depending on the solvent media. I...

The chemiluminescence (CL) accompanying the oxidation of phenyl hydrazine with t-butylhydroperoxide in alkaline medium was studied in the presence and absence of luminol. The reaction occurring between phenyl hydrazine and alkaline (KOH) solution of aqueous tbutylhydroperoxide leads to the production of nitrogen gas with simultaneous emission of light (CL), which has been detected by PMT and re...

Fluorescence (FL), chemiluminescence (CL) and bioluminescence (BL) have been extensively focused on luminescence assays. Various FL and CL compounds have been synthesized and evaluated their FL and CL properties. This review describes development of highly selective and sensitive FL and CL reagents by derivatizing luminol, indole and polyphenol. Luminol derivatives were used for measuring enzym...

Luminol electrochemiluminescence is dramatically increased by about five hundred times by taking full advantage of both electrochemical reduction and electrochemical oxidation using simple linear sweep voltammetry, leading to sensitive detection.

The spontaneous emission of chemiluminescence appears to reflect changes in the intracellular steady-state concentration of oxygen radicals. Stimulation of arachidonic acid metabolism in human platelets also induces chemiluminescence, which indicates the formation of highly reactive species by cyclooxygenase andlor lipooxygenase [1,2]. It was observed that arachidonic acid, linoleic acid and li...

Based on a new electrogenerated chemiluminescence (ECL) analytical idea, this paper explains a sensitive and selective flow-injection ECL method using luminol for the determination of isoniazid, based on the sensitizing effect of isoniazid for the weak ECL emission of electrochemically oxidized luminol. Under the optimum experimental conditions, the relative ECL intensity was linear with isonia...