The presence of acid fast bacilli in sputum specimen was investigated comparatively with Ziehl-Neelsen (ZN)and Kinyoun Cold staining (KC) in order to determine sensitivity in detecting Tuberculosis Bacilli (T.B.). This study was employed on patients with severe lower respiratory tract infection to compare between these two stains. Which revealed that Ziehl-Neelsen procedure gives better results...

OBJECTIVE To evaluate the performance of GeneXpert for detection of mycobacterium tuberculosis in pus samples and compare its results with conventional techniques in terms of validity, rapidity and rifampicin resistance. METHODS This longitudinal, descriptive study was conducted at Jinnah Hospital, Lahore, Pakistan, from January 2012 to December 2015, and comprised pus samples of people suspe...

Infeksi protozoa merupakan salah satu penyakit parasitik yang umumnya bersifat kronis serta menyebabkan penurunan berat badan. Penelitian ini bertujuan untuk mengetahui gastrointestinal menginfeksi orangutan sumatera (Pongo abelii) dan kalimantan pygmaeus) berada di Taman Satwa Sumatera Utara dari genus apa saja tersebut. Sampel pada penelitian menggunakan sampel feses didapat 1 individu 2 kali...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...

BACKGROUND The main goal of the present study was to develop a new sensitive and specific PCR based method for Identification of Cryptosporidium sp. using novel primers from 18S ribosomal RNA. Cryptosporidiosis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. To the best of our knowledge this is the first study regarding develop a new PCR...

Fluorescence microscopy for detecting mycobacteria was first used by Hagemann (1) in 1937. Further publications are rare . This method is employed more commonly in smears ( I. 2. 4. 5. K. 12) and rarely in tissue sections (9. 11 ). It gives better results in comparison with the Ziehl-Neel sen stain in both types of material. I n sections of lesions due to leprosy, fluorescence microscopy has no...

microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...