The enzyme L-Amino Acid Oxidase (LAAO) is widely distributed in snake venoms, contributes a reasonable toxicity to the venoms. However LAAO from Indian cobra (Naja naja ) snake venom has not yet isolated previously. In the present study LAAO from Indian cobra (Naja naja ) snake venom was purified by sequential steps of cation exchange chromatography (CM-Sephadex C-25; CM-52 cellulose), followed...

Background: Antibacterial proteins are widely expressed in snake venoms. Previously, we have isolated two immunodominant proteins with molecular weights of 14 and 65kD from the snake venom of Naja naja oxiana (N. oxiana). It was demonstrated that they had potent inhibitory effects against gram-positive bacteria, S. aureus and B. subtilis but were less effective against gram-negative bacteria, s...

Introduction: The design and structural characterization of mini-proteins with a compact, folded structure provide insight into the complex architecture of proteins today and has long been a challenging issue in structural- functional studies. Alpha neurotoxins from snake venom have a distinct folded structure comprised of a disulphide core and three loops or “fingers” each of these loops are c...

Cytogenetic studies were carried out on four species of Indian snakes viz Eryx conicus (Family Boidae), Naja naja naja (Family Elapidae), Vipera russelli and Echis carinatus (Family Viperidae) collected from Jammu region (J&K). Both E. conicus and N. n. naja show deviation from typical ophidian karyotype (2n=36), having 2n=34 (16 macroand 18 microchromosomes, NF=42) in E. conicus and 2n=38 (16 ...

one small-scale double emulsion technique for incorporation of naja- naja oxiana venom into poly (lactide-co-glycolide) (plga) microspheres were developed and optimized. the effects of high speed homogenization on the double emulsion stability, microsphere size, entrapment efficiency and in vitro release of venom were studied. a stable double emulsion was verified by homogenization method. slow...

The secondary structures of three snake venom cardiotoxins (from Hemachatus hemachatus, Naja naja atra, and Naja naja naja), in aqueous solution and in a lipid-bound form, were investigated by Fourier-transform infrared spectroscopy. The conformation-sensitive protein infrared bands in the amide I region were analyzed using deconvolution and band-fitting procedures. The spectra of the three car...