ells with shortening telomeres hit two successive barriers: they first stop dividing as they become senescent, and later undergo a cell death process termed crisis. Although crisis may, as suggested by others, be controlled by overall telomere length, and colleagues now blame the onset of senescence on a particular structure at the end of telomeres. The important structure is a single-stranded ...

Although telomeres are not recognized as double-strand breaks (DSBs), some DSB repair proteins are present at telomeres and are required for telomere maintenance. To learn more about the telomeric function of proteins from the homologous recombination (HR) and non-homologous end joining pathways (NHEJ), we have screened a panel of chicken DT40 knockout cell lines for changes in telomere structu...

Mammalian telomeres contain a duplex TTAGGG-repeat tract terminating in a 3' single-stranded overhang. TRF2 protein has been implicated in remodeling telomeres into duplex lariats, termed t-loops, in vitro and t-loops have been isolated from cells in vivo. To examine the features of the telomeric DNA essential for TRF2-promoted looping, model templates containing a 500 bp double-stranded TTAGGG...

This paper summarizes a theoretical and an experimental approach to employ the Direct Metal Deposition (DMD) process to adjudge the feasibility of depositing overhang features. The DMD process is a process capable of directly manufacturing a fully dense metal part without the use of any intermediate steps. This process, comprised of a laser deposition setup coupled with a 5-axis CNC milling sys...

PURPOSE To compare the incidence of overhang between two distinct femoral components and whether there is clinical and radiological benefit of gender-specific implants in short-term follow-up. MATERIALS AND METHODS One hundred and four knees in consecutive 66 female patients who underwent primary total knee arthroplasty due to primary osteoarthritis were included in this study. Overhang was m...