BglII 5’...A_GATCT...3’ (5’overhang) BamHI 5’...G_GATCC...3’ (5’overhang); notice: MboI and Sau3AI = 5’..._GATC...3’ EcoRI 5’...G_AATTC...3’ (5’ overhang) HpaII 5’...C_CGG...3’ (5’ overhang); notice: CpG methylation blocked HindIII 5’...A_AGCTT...3’ (5’ overhang) KpnI 5’...GGTAC_C...3’ (3’ overhang) NcoI 5’...C_CATGG...3’ (5’ overhang); notice: Kozak sequence (RCC)ACCATGG NotI 5’...GC_GGCCGC......

Introdouction: Overhang refers to the extension of restoration material from the cavity. It has an important role in decay, plaque accumulation and periodontal disease, so the aim of this study is determining the frequency of restoration overhang in patients in Anzali, Guilan. Materials and methods: This descriptive cross-sectional study was completed using 293 patients who visited fo...

introdouction: overhang refers to the extension of restoration material from the cavity. it has an important role in decay, plaque accumulation and periodontal disease, so the aim of this study is determining the frequency of restoration overhang in patients in anzali, guilan. materials and methods: this descriptive cross-sectional study was completed using 293 patients who visited for routine ...

Non-homologous end joining (NHEJ) is a major DNA double-strand break (DSB) repair mechanism. We characterized here a series of plasmid-based DSB templates that were repaired in Xenopus egg extracts via the canonical, Ku-dependent NHEJ pathway. We showed that the template with compatible ends was efficiently repaired without end processing, in a manner that required the kinase activity of DNA-PK...

Background: The use of daylight in offices has become an effective strategy to improve the quality of indoor space and energy efficiency. There is enough potential to have daylight autonomy in Kerman as a city with more than 300 sunny days a year. Therefore, this study aimed to investigate the relationship between facades configuration and the amount of daylight in office buildings of Kerman. ...

PURPOSE The purpose of the present study is to compare the prevalence of implant overhang between the Oxford and the Miller-Galante II (M-G II) unicompartmental knee arthroplasty (UKA) prostheses and determine whether overhang is associated with postoperative clinical results. MATERIALS AND METHODS We retrospectively reviewed one hundred and seven UKAs which consisted of 37 Oxford UKAs and 70...

Enhancing the ventilation performance of energy-efficient buildings with single-sided openings is important because their ventilation performance is poor and strongly depends on the wind conditions. We considered an overhang as a potential building façade for improving the single-sided ventilation performance. We performed numerical simulations of three-dimensional unsteady turbulent flows over...

INTRODUCTION: Tibial implant overhang has been identified as a possible problem in unicompartmental knee replacements (UKR) and to some degree in total knee replacements (TKR). The idea of an optimally placed tibial implant is that the implant would be well supported by the cortical rim of the proximal tibia without overhanging. Overhang on the medial side can cause irritation of soft tissues a...

For powder-bed electron beam additive manufacturing (EBAM), support structures are required when fabricating an overhang to prevent defects such as curling, which is due to the complex thermomechanical process in EBAM. In this study, finite element modeling is developed to simulate the thermomechanical process in EBAM in building overhang part. Thermomechanical characteristics such as thermal g...