Autotransporters are bacterial virulence factors that consist of an N-terminal extracellular ("passenger") domain and a C-terminal β barrel domain ("β domain") that resides in the outer membrane. Here we used an in vivo site-specific photocrosslinking approach to gain insight into the mechanism by which the β domain is integrated into the outer membrane and the relationship between β domain ass...

Escherichia coli maltose binding protein (MBP) is commonly used to promote the solubility of its fusion partners. To investigate the mechanism of solubility enhancement by MBP, we compared the properties of MBP fusion proteins refolded in vitro with those of the corresponding fusion proteins purified under native conditions. We fused five aggregation-prone passenger proteins to 3 different N-te...

Cruising as part of nautical tourism is an important segment maritime passenger transportation. Nautical and cruise have developed over the years are experiencing increasing demand, with exception pandemic period, when did not experience positive trends. In normal conditions, high growth requires increase in supply, which can negative consequences for environment. This especially true destinati...

BACKGROUND Gram-negative bacteria have developed a limited repertoire of solutions for secreting proteins from the cytoplasmic compartment to the exterior of the cell. Amongst the spectrum of secreted proteins are the intimins and invasins (the Int/Inv family; TC# 1.B.54) which are characterized by an N-terminal β-barrel domain and a C-terminal surface localized passenger domain. Despite the im...

Autotransporters are secreted virulence factors that comprise three domains: an N-terminal signal peptide, an internal passenger domain, and a C-terminal beta-domain. The mechanism of passenger translocation across the outer membrane remains undefined, with four models having been proposed: the "hairpin," the "threading," the "multimeric," and the "Omp85 (YaeT)" models. In an attempt to underst...

This paper explores the need, development of computerized passenger reservation system for Indian railways. The passenger reservation system is based on online transaction processing. It was given the name Integrated Multitrain Passenger REServation System (IMPRESS) which later developed into single image system called Countrywide Network Of Computerized Enhanced ReservaTion (CONCERT). Then thi...