This study aimed to determine the effects of vitamin C supplementation on antioxidant and inflammatory status cell viability bovine peripheral blood mononuclear cells. Peripheral cells (PBMCs) were isolated from eighteen clinically healthy Japanese Black calves in this study. PBMCs cultured with (vitamin group) without (control stimulated or lipopolysaccharide (LPS). As a result, total capaciti...

The concentration of IgG and IgA was measured in the supernatants of peripheral blood mononuclear cells and of cells harvested from the intestinal lamina propria, which were cultured in vitro in the presence or absence of mitogens. The lamina propria mononuclear cells were harvested by collagenase digestion of macroscopically normal mucosa from 10 fresh surgical resections for carcinoma. Secret...

in this center, from 1991 to 2002, 89 chronic myelogenous leukemic (cml) patients, age ranging between 8-48 years with a median age of 29, underwent hematopoietic stem cell transplantation. eighty-eight patients were in the first chronic phase of disease. twenty-three patients received bone marrow transplantation (bmt) and 66 patients received peripheral blood stem cell transplantations (pbsct)...

Background: Atherosclerosis, a chronic inflammatory disease of the vessel wall is characterized by local and systemic immune responses to a variety of antigens. Oxidized low-density lipoprotein (oxLDL) is considered as an important determining factor in the pathogenesis of atherosclerosis. Objective: The purpose of this study was to investigate the degree of peripheral blood mononuclear cells (...

To produce an in vitro model of nemaline myopathy, we reprogrammed the peripheral blood mononuclear cells (PBMCs) a patient with heterozygous p.Gly148Asp mutation exon 3 ACTA1 gene to iPSCs. Using CRISPR/Cas9 editing corrected generate isogenic control line. Both mutant and show normal karyotype, express pluripotency markers could differentiae into three cell states that represent embryonic ger...

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...