Although the results of isotopic experiments (Sacks & Altschuler, 1942; Harvey, 1955) had been interpreted as indicating the possible presence in muscle of a synthetic pathway for the formation of Nphosphorylcreatine not involving the adenine nucleotides, there was no direct evidence for such a reaction until the report by Cori, Abarca, Frenkel & Traverso-Cori (1956). These authors claimed that...

Microsomal preparations from the developing cotyledons of safflower (Carthamus tinctorius) catalyse the acylation of sn-glycerol 3-phosphate in the presence of acyl-CoA. Under these conditions the radioactive glycerol in sn-glycerol 3-phosphate accumulates in phosphatidic acid, phosphatidylcholine, diacyl- and tri-acylglycerol. The incorporation of glycerol into phosphatidylcholine is via diacy...

In the present study, application of porous acidic catalyst functionalized with an imidazoleionic liquid ([SBA-Im]HSO4) as a phase transfer catalyst for the facile preparation of benzylthiocyanates and azides in water has been described. The catalyst has been characterized byFourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), transmissionelectron microscopy (TEM...

Nanoparticles introduced in living cells are capable of strongly promoting the aggregation of peptides and proteins. We use here molecular dynamics simulations to characterise in detail the process by which nanoparticle surfaces catalyse the self-assembly of peptides into fibrillar structures. The simulation of a system of hundreds of peptides over the millisecond timescale enables us to show t...

A set of six multinuclear manganese complexes was screened for the ability to catalyse reactions yielding O(2) under coherent experimental conditions; we identify a much larger number of manganese compounds than previously known that catalyse oxygen formation.

in order to simulate the rotating disk contactor all hydrodynamic parameters, dispersed phase hold-up, characteristic velocity, mean drop size, drop size distribution, axial mixing and mass transfer have been taken into account. mechanisms of mass transfer (stagnant, circulating, oscillating drops) were used in this study. column height found from effect of mean drop size and drop size distribu...

Several mechanisms have been proposed for the synthesis of substrate-linked ubiquitin chains. HECT ligases directly catalyse protein ubiquitination and have been found to non-covalently interact with ubiquitin. We report crystal structures of the Nedd4 HECT domain, alone and in complex with ubiquitin, which show a new binding mode involving two surfaces on ubiquitin and both subdomains of the H...

F-type H+-ATP synthases synthesize ATP from ADP and phosphate using the energy supplied by a transmembrane electrochemical potential difference of protons. Rotary subunit movements within the enzyme drive catalysis in either an ATP hydrolysis or an ATP synthesis direction respectively. To monitor these subunit movements and associated conformational changes in real time and with subnanometre re...

Membrane contactor using amine based absorbents is an efficient technology for CO2 separation from gaseous mixtures. A novel porous polysulfone (PSF) flat membrane was prepared via non-solvent phase inversion method. The PSF membrane was modified by adding polyvinyl pyrrolidone (PVP) to the dope solution. The fabricated membrane was used in the serpentine flow field contactor module for CO2 abs...