We report a PCR-induced artifact in testing for homologous recombination in zebrafish. We attempted to replace the lnx2a gene with a donor cassette, mediated by a TALEN induced double stranded cut. The donor construct was flanked with homology arms of about 1 kb at the 5' and 3' ends. Injected embryos (G0) were raised and outcrossed to wild type fish. A fraction of the progeny appeared to have ...

β-thalassemia, a monogenic autosomal recessive disorder, is prevalent in middle east, particularly in iran. in iran, near to 20 mutations in the β-globin gene are introduced as common mutations with varying incidence frequencies in each city. therefore, detection and screening for couples at high risk can help to solve the problems of this disease. in this study, optimized genotyping of two com...

The fifth component of complement (C5) is considered to be the center of complement activation and function. However, there are no genetically engineered knockout mice for this gene, and the only commercially available inherited C5-deficient mice, in which a "TA" nucleotide deletion in the coding frame was previously identified, are in theC57BL/10Sn genetic background rather than the commonly u...

Sugarcane is an important sugar and energy crop in the world. Germplasm innovation a significant way to breed breakthrough sugarcane varieties. Modern varieties all contain blood relationship of Saccharum officinarum spontaneum. High results from S. resistance genes In order improve quality, breeders use spontaneum cross obtain hybrid offspring with high resistance. Therefore, authenticity prog...

background: leishmaniasis is a tropical disease that is endemic in some areas of iran, including east azerbaijan . ifn-γ is one of the cytokines that triggers cell-mediated immunity, thus initiating elimination of the infection. this case-control study was performed to investigate the association between the polymorphism of the ifn-γ gene at the +874a/t locus and visceral leishmaniasis (vl). me...