نتایج جستجو برای: primer arms pcr

تعداد نتایج: 224806  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه اصفهان - دانشکده علوم 1391

سرطان کولورکتال شامل سرطان در ناحیه ی کولون، رکتوم و زائده ی آپاندیس است. سرطان کولون و رکتوم خصوصیات مشترک زیادی با یکدیگر داشته، به همین دلیل اغلب آنها را با هم در نظر گرفته و به عنوان سرطان کولورکتال شناسایی می-شوند. در ایران سالیانه حدود 2262 نفر در اثر ابتلا به این سرطان جان خود را از دست می دهند. سرطان ابتدا به صورت پولیپ-های خوش خیم ظاهر می گردد که می تواند به سرطان تهاجمی و کشنده تبدیل ...

Journal: :basic and clinical cancer research 0
majid motovali-bashi genetics division, department of biology, faculty of science, university of isfahan, isfahan, iran zahra sadeghi genetics division, department of biology, faculty of science, university of isfahan, isfahan, iran simin hemati department of radiotherapy and oncology, faculty of medicine, isfahan university of medical sciences, isfahan, iran

background and objective: mmp enzymes are a family of membrane proteins that are capable of digesting extracellular matrix compounds (ecm) and basement membrane. matrilysin enzyme is the smallest member of mmp family that is encoded by mmp-7 gene (matrilysin). according to the reports, g allele of -181 a/g single nucleotide polymorphism of mmp-7 gene causes an increase in the expression of this...

2012
Andrew Collins Xiayi Ke

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...

Journal: :Nucleic acids research 2001
S Ye S Dhillon X Ke A R Collins I N Day

Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pa...

2014
Sonika Ahlawat Rekha Sharma A. Maitra Manoranjan Roy M.S. Tantia

New, quick, and inexpensive methods for genotyping novel caprine Fec gene polymorphisms through tetra-primer ARMS PCR were developed in the present investigation. Single nucleotide polymorphism (SNP) genotyping needs to be attempted to establish association between the identified mutations and traits of economic importance. In the current study, we have successfully genotyped three new SNPs ide...

ژورنال: :مجله علوم پزشکی رازی 0
مجید متولی باشی majid motovali-bashi university of isfahan, isfahan, iranدانشگاه اصفهان، اصفهان، ایران سروش کرد soroush kord university of isfahan, isfahan, iranدانشگاه اصفهان، اصفهان، ایران

زمینه و هدف: تالاسمی اینترمدیا یک بیماری اتوزوم مغلوب بوده که از نظر بالینی طیفی بسیار گسترده­ از اختلالات وابسته به هموگلوبین را شامل می شود و از نظر شدت بیماری بین تالاسمی ماژور و مینور قرار می­گیرد. سطح بالای هموگلوبین جنینی تاثیر عمده­ای بر وخامت کلینیکی (بالینی) این بیماری دارد، به طوری که افزایش تولید hbf شدت بیماری را کاهش می­دهد. عوامل مختلفی درون لوکوس بتا گلوبین می­توانند در کاهش شدت ...

Journal: :Genetics and molecular research : GMR 2013
P A S Fonseca I C Rosse M Demiranda M A Machado R S Verneque M G C D Peixoto M R S Carvalho

Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduce...

2013
Chen Zhang Ying Liu Brian Z. Ring Kai Nie Mengjie Yang Miao Wang Hongwei Shen Xiyang Wu Xuejun Ma

BACKGROUND The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capil...

Journal: : 2023

Mục tiêu nghiên cứu: Xây dựng quy trình phát hiện và bước đầu khảo sát điểm đa hình đơn nucleotide (SNP) rs7228049 trên gen SOCS6 người Việt Nam bằng phương pháp Tetra-primer ARMS PCR (T-ARMS PCR). Đối tượng Điểm gene được T-ARMS PCR, có đối chiếu với kết quả giải tự Sanger 120 khỏe mạnh. Kết quả: Đã các kiểu đồng hợp tử AA, GG dị GA tại SNP kỹ thuật PCR. Trên nhóm mạnh, tần suất alen G A lần l...

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