METHODS. Glutathione-S-transferase (GST) fusion proteins containing two SH2 domains of the p85 regulatory subunit of PI3K—GST-p85 (N-SH2), GST-p85 (C-SH2), and respective SH2 mutants (N-SH2, R358A, and C-SH2, R649A)—were prepared and used to pull down tyrosine-phosphorylated proteins in bovine ROS. Protein identity was established by Western blot analysis. PI3K activity was determined in the pu...

AbstructA novel BiCMOS full-swing circuit technique with superior performance over CMOS down to 1.5 V is proposed. A conventional noncomplementary BiCMOS process is used. The proposed pull-up configuration is based on a capacitively coupled feedback circuit. Several pull-down options were examined and compared, and the results are reported. Several cells were implemented using the novel circuit...

Arm and hand use by the mouse have been studied in a variety of tasks in order to understand the structure of skilled movements and motor learning, the anatomy and function of neural pathways, and to develop animal models of neurological conditions. The present study describes string-pulling by the mouse, a behavior in which a mouse uses hand-over-hand movements to pull down a string that hangs...

2556-Pos Board B575 Bringing Deconvolution Algorithmic Techniques to the Electron Microscope Ben Lich, Xiaodong Zhuge, Pavel Potocek, Faysal Boughorbel, Cliff Mathisen. FEI Company, Eindhoven, Netherlands. The Scanning Electron Microscope (SEM) as a three dimensional imaging tool is increasingly popular for studies in neurobiology. Serial slicing methods based on diamond-knife cutting are, howe...

Previous studies have employed various models for exploring menu use, focusing mainly on short menus. In this study, long pull-down menus were examined in an item selection experiment. Our base model assumed that 1) the first eye fixation was located at the top, middle, or end of a menu depending on the search item; 2) menu scanning is either downward or upward; 3) the mouse was slowly moved do...

Proteomics and high throughput analysis for systems biology can benefit significantly from solid-phase chemical tools for affinity pull-down of proteins from complex mixtures. Here we report the application of solid-phase synthesis of phosphopeptides for pull-down and analysis of the affinity profile of the integrin-linked kinase associated phosphatase (ILKAP), a member of the protein phosphata...

Synthesis of dynamic combinatorial libraries acylhydrazones in the presence a G-quadruplex DNA template, followed by pull-down with streptavidin-coated magnetic beads, allows identification putative binders.