In the title compound, C(14)H(14)F(3)NO(4), the 3,3,3-trifluoro-pyruvate fragment has a syn configuration and is noncoplanar with the indole plane [dihedral angle = 84.87 (5)°]. In the crystal, mol-ecules form inversion-related dimers via pairs of inter-molecular O-H⋯O hydrogen bonds. These dimers are connected by inter-molecular N-H⋯O=C(CF(3)) hydrogen bonds to form a two-dimensional network s...

Hyperpolarized (13)C imaging allows real-time in vivo measurements of metabolite levels. Quantification of metabolite conversion between [1-(13)C]pyruvate and downstream metabolites [1-(13)C]alanine, [1-(13)C]lactate, and [(13)C]bicarbonate can be achieved through kinetic modeling. Since pyruvate interacts dynamically and simultaneously with its downstream metabolites, the purpose of this work ...

Optimal reaction conditions to sassay human lactate dehydrogenase (lactate-to-pyruvate) were established for isoenzymes 1 and 5 at 25, 30, and 37 degrees C in diethanolamine and 2-amino-2-methyl-1,3-propanediol. Different substrate concentrations are required at each temperature. The conditions permit measurement of lactate dehydrogenase 1 and 5 with the lowest substrate concentrations that all...

The 4-hydroxy-4-methyl-2-oxoglutarate (HMG)/4-carboxy-4-hydroxy-2-oxoadipate (CHA) aldolase is the last enzyme of both the gallate and protocatechuate 4,5-cleavage pathways which links aromatic catabolism to central cellular metabolism. The enzyme is a class II, divalent metal dependent, aldolase which is activated in the presence of inorganic phosphate (Pi), increasing its turnover rate >10-fo...

DONALD E. PAGLIA, WILLIAM N. VALENTINE AND DONALD L. RUCKNAGEL with the technical assistance of MARY Lu WILSON and JULIE WITTENBERG Departments of Medicine and Pathology, Centerfor the Health Sciences, University of California, Los Angeles; Research and Medical Services, Veterans Administration Center, Los Angeles, Culifornia; and Department of Human Genetics, University of Michigan Medical Sch...

Pyruvate kinase and phosphoenolpyruvate, added to actomyosin, cause a maintenance of the response of the actomyosin to stoichiometric amounts of ATP. This steady state maintenance depends on the presence of Mg ions.

In the title complex, [Sn(2)(C(7)H(7))(4)(C(10)H(8)N(2)O(3))(2)(C(2)H(5)OH)(2)], the Sn(IV) atom is seven-coordinated in a distorted penta-gonal-bipyramidal geometry by three O atoms and one N atom from the pyruvate benzoyl hydrazone ligand, one ethanol O atom and two axial C atoms from trans-benzyl groups, thus forming a dimeric mol-ecule ([Formula: see text] symmetry) via weak Sn-O inter-acti...