BACKGROUND Typing of nosocomial pathogens is necessary to determine the source of an outbreak. The aim was to determine the genomic variability among Pseudomonas aeruginosa (P. aeruginosa) by random amplification of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) methods. METHODS Fifty P. aeruginosa isolates were obtained from the hospitals. The source of the...

Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based –PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtai...

RAPD is a PCR based technique which involves the use of single arbitrary. Advantages disadvantages, limitations and applications of RAPD.RAPD is a modification of the PCR in which a single, short and arbitrary. Therefore, RAPD technique can be performed in a moderate laboratory for most of.sophisticated equipment has made the RAPD technique valuable although. Tartflmann odak noktas ise de, RAPD...

Background: Due to the increasing antibiotic resistance, treating infections caused by Klebsiella pneumoniae has become more challenging. Objectives: The present study aimed investigate prevalence of blaOXA-48 and blaNDM producing carbapenem-resistant K. isolated from clinical samples in Shahid Rajaei hospital Tehran, Iran. Methods: Various were collected 1,186 patients admitted with open heart...

Abstract Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus genus that includes heterogeneous species, thus combination techniques essential for their identification. Here we RAPD-PCR methodology distinguish among genetically similar strains and evaluate the genetic diversity species from Salar...

PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) has been used widely for genome identification. In this study, 13 Salmonella Typhi strains were isolated from typhoid patients from Aswan, Cairo, Fayoum, and Monofya Governorates of Egypt. The isolates, along with three reference strains, i.e., O901, H901, and Ty2 were subjected to whole genome typing by RAPD PCR. Three RAPD...

PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discrim...

Random-amplified-polymorphic-DNA(RAPD) was assayed to detect the genetic variation of 6 barley generations from Iraq. Four primers generated a total 17 scoreable bands in RAPD analysis) and resolving power, three polymorphic differed (Rp). The use marker systems distance among generation discovered be beneficial. dendrograms indicate diverse grouping barely specimens, although we did see that c...

This study was conducted to determine whether there was any genetic heterogeneity among Helicobacter pylori strains isolated from the antrum and corpus of the same individual in a Malaysian population and to determine the presence of heterogeneous susceptibility of the isolates by comparing PCR-RAPD and antibiotic profiles. Forty-four H. pylori isolates cultured from the antrum and corpus of 22...