نتایج جستجو برای: recombinant coat protein

تعداد نتایج: 1310399  

Journal: :iranian journal of biotechnology 2006
mojgan bandehpour negar seyed mehdi shadnoush parviz pakzad bahram kazemi

chlamydia trachomatis is one of the main causes of sexually transmitted diseases (stds) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of chlamydia. in this research the chlamydia major o...

Journal: :avicenna journal of medical biochemistry 0
fatemeh ebrahimzadeh research center for molecular medicine, department of molecular medicine and genetics, hamadan university of medical sciences, hamadan, ir iran yeganeh talebkhan department of biotechnology, biotechnology research center, pasteur institute of iran, tehran, ir iran hassan mirzahoseini department of biotechnology, biotechnology research center, pasteur institute of iran, tehran, ir iran ghasem barati research center for molecular medicine, department of molecular medicine and genetics, hamadan university of medical sciences, hamadan, ir iran massoud saidijam research center for molecular medicine, department of molecular medicine and genetics, hamadan university of medical sciences, hamadan, ir iran; research center for molecular medicine, department of molecular medicine and genetics, school of medicine, hamadan university of medical sciences, hamadan, ir iran. tel: +98-9121324616, fax: +98-8138380464

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

Journal: :archives of clinical infectious diseases 0
bahareh hajikhani department of bacteriology, faculty of medical sciences, tarbiat modarres university, tehran, ir iran shahin najar peerayeh department of bacteriology, faculty of medical sciences, tarbiat modarres university, tehran, ir iran; department of bacteriology, faculty of medical sciences, tarbiat modarres university, jalal ale ahmad highway, tehran, ir iran. hoorieh soleimanjahi department of virology, faculty of medical sciences, tarbiat modarres university, tehran, ir iran zuhair m hassan department of immunology, faculty of medical sciences, tarbiat modarres university, tehran, ir iran gholamreza goudarzi department of microbiology, lorestan university of medical sciences, khorramabad, ir iran

conclusion our findings confirmed that a prokaryotic expression system of rureb229-561 was successfully constructed.the results of sds-page showed that our constructed prokaryotic expression system pet28a- ureb229-561 -bl21de3 efficiently produces target recombinant protein in the form of dissoluble inclusion body. therefore we can suggest that these epitopes can effectively be a vaccine candid...

Journal: :iranian journal of immunology 0
bahram kazemi cellular and molecular biology research center negar seyed cellular and molecular biology research center mojgan bandehpour cellular and molecular biology research center zarrin sharifnia cellular and molecular biology research center parviz pakzad department of immunology, shahid beheshti university of medical sciences, tehran, iran

background: although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. objective: the aim of this study was to clone the first 1100 bp of the c. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...

Journal: :iranian journal of basic medical sciences 0
mahrou sadri blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran department of medical biotechnology, faculty of allied medicine, iran university of medical sciences, tehran, iran mohammad m farajollahi department of medical biotechnology, faculty of allied medicine, iran university of medical sciences, tehran, iran zohreh sharifi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran

objective(s): hepatitis c is a major cause of liver failure worldwide. current therapies applied for this disease are not fully effective and produce side effects in most cases. non-structural protein 3 helicase (ns3) of hcv is one of the key enzymes in viral replication and infection. therefore, this region is a promising target to design new drugs and therapies against hcv infection. the aim ...

Journal: :iranian journal of basic medical sciences 0
khadijeh nasiri department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad , iran saeed zibaee razi vaccine and serum research institute, agricultural research, education and extension organization (areeo), mashhad, iran mohammadreza nassiri department of animal science and institute of biotechnology, ferdowsi university of mashhad, mashhad, iran mojtaba tahmoorespur department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad , iran alireza haghparast department of veterinary medicine, faculty of veterinary medicine, ferdowsi university of mashhad, mashhad, iran

objective(s): enterotoxigenic escherichia coli (etec) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. igy technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. the purpose of this study was to produce specific antibody by egg yolk antibody (igy...

Tobacco mosaic virus (TMV) is one of the devastating plant viruses in the world that infects more than 200 plant species. Movement protein plays a supportive role in the movement of other plant viruses, and viral coat protein is highly expressed in infected plants and affects replication and movements of TMV. In order to investigate genetic variation in the terminal fragment sequence in Iranian...

Journal: :Indian journal of experimental biology 2008
M Hema Ch V Subba Reddy H S Savithri P Sreenivasulu

Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by s...

Journal: :The Journal of general virology 1991
D J Bertioli R D Harris M L Edwards J I Cooper W S Hawes

The 3' end of the RNA-2 of arabis mosaic virus (ArMV) was cloned and sequenced. The N-terminal amino acid sequence of the virion coat protein was determined by Edman degradation and the corresponding coding region identified. This gene was modified at the 5' and 3' ends by use of mismatched primers in the polymerase chain reaction (PCR), in order to facilitate the cloning of the gene, and to pr...

Journal: :Cytotechnology 2000
H K Ong A M Ali A R Omar K Yusoff

The haemagglutinin-neuraminidase (HN) gene ofNewcastle disease virus (NDV) strain AF2240, amplifiedfrom the viral genomic RNA ( approximately 1.8 kb) was directionallycloned and inserted into a baculovirus expressionvector system. The recombinant glycoprotein expressedin Spodoptera frugiperda (Sf9) cellsshowed haemagglutinin (HA), neuraminidase (NA) andhemadsorption activities. HA activity was ...

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