نتایج جستجو برای: rep and box primers
تعداد نتایج: 16839713 فیلتر نتایج به سال:
introduction and aims: h. pylori has been implicated in peptic diseases, some with detrimental consequences such as ulcer or cancer. since considerable genetic heterogeneity has been observed within h. pylori population worldwide, it appears an ideal achievement to recruit pcr-based methods and design genetic markers which recognize isolates from normal and symptomatic individuals. in this stud...
Repetitive-element PCR (rep-PCR) fingerprinting is a promising molecular typing tool for Escherichia coli, including for discriminating between pathogenic and nonpathogenic clones, but is plagued by irreproducibility. Using the ERIC2 and BOXA1R primers and 15 E. coli strains from the ECOR reference collection (three from each phylogenetic group, as defined by multilocus enzyme electrophoresis [...
توسکا یکی از مهم ترین گونه های درختی جنگل های شمال کشور بوده و از نظر اقتصادی به عنوان چهارمین درخت تجاری محسوب می شود. یک بیماری لکه برگی ناشی از گونه ای زانتوموناس در توسکا ییلاقی (alnus subcordata subsp. subcordata) در چند منطقه جنگلی استان مازندران گزارش شده است (rahimian et al. 1383. 16th iran. plant protec. cong.,439). براساس یک بررسی ژنومی که در سال های اخیر انجام شده بود باکتری عامل بیم...
Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method. Methods:...
باکتری pseudomonas syringae pv. syringae عامل بیماری های متعددی در بیش از 180 گونه گیاهی متعلق به جنس های مختلف است. در سال های اخیر بیماری سوختگی باکتریائی یونجه در مناطق مختلف استان کردستان گسترش یافته است. خصوصیات فیزیولوژیکی و بیوشیمیائی 64 جدایه مورد بررسی قرار گرفت. خصوصیات بیش از 80% جدایه های مورد مطالعه مشابهpseudomonas syringae pv. syringae lopat group 1a و کمتر از 5% جدایه ها مشابه l...
چکیده rathtayibacter spp. عامل بیماری خوشه صمغی روی گیاهان مختلف خانواده گندمیان می باشد. از مهم ترین میزبان های این باکتری، که در پاره ای از نقاط خسارت قابل توجهی را تحمل می کنند ، گندم (triticum aestivum) و جو (hordeum vulgare) اند. در سال های 1382-1381 نمونه برداری از مزارع گندم و جو واقع در مناطق مختلف استان های آذربایجان شرقی، فارس، ایلام، اصفهان و خوزستان صورت گرفت. علائم بیماری به صورت ...
during 2007-2008, 58 strains of p. syringae pv. syringae (pss) were isolated from various prunus species and other hosts such as sugar beet, pear, quince, oat, millet, wheat, barley, and rice in fars, isfahan, kohgiloye and boyer ahmad, chahar mahal-o-bakhtiari provinces of iran. the strains were tested for pathogenicity, the presence of the syrb gene and box pcr (box a1r primer). all teste...
Background: Salmonellosis is a zoonotic disease of human and animals. One of the most mportant serovars in human, livestock and poultry is Salmonella enteritidis which is a worldwide common foodborne pathogen. The aim of this study was differentiation and to determine the relationship among different isolates of Salmonella enteritidis by molecular fingerprinting method based on repeated sequenc...
Polymerase chain reaction (PCR) with degenerate primers was utilized for partial cloning of the MADS box gene family from wheat (Triticum aestivum L.). PCR products corresponding to a part of the MADS box region were cloned and sequenced. Eleven individual clones sequenced were classified into seven types on the basis of the nucleotide sequence and five types on the deduced amino acid sequence,...
Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based -PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtai...
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