Three different experiments were conducted to study the effect of dietary astaxanthin on the immune response, resistance to white spot syndrome virus (WSSV) and transcription of antioxidant enzyme genes in Pacific white shrimp Litopenaeus vannamei. Each experiment included one triplicate treatment group (fed with 80 mg/kg astaxanthin supplemented diet) and one triplicate control group (fed with...

The observation that the activity of sialyltransferase (EC 2.4.99.1; serum glycoprotein:N-acetylneuraminic acid transferase) is often elevated in the serum of cancer patients necessitates an elucidation of the interrelationships of this serum enzyme with host tissues. Accordingly, the activity of this enzyme in serum, tumor, and liver was determined at various times after implantation of the R3...

Three different experiments were conducted to study the effect of dietary astaxanthin on the immune response, resistance to white spot syndrome virus (WSSV) and transcription of antioxidant enzyme genes in Pacific white shrimp Litopenaeus vannamei. Each experiment included one triplicate treatment group (fed with 80 mg/kg astaxanthin supplemented diet) and one triplicate control group (fed with...

Q fever (coxiellosis) is a zoonotic infection caused by the bacterium Coxiella (C.) burnetii that can infect many species of mammals, including humans. This work was aimed at studying prevalence C. among livestock and people in southern region Kazakhstan using enzyme-linked immunosorbent assay (ELISA). For serological monitoring, serum samples were collected from 100 residents Zhambyl oblast ep...

conclusions our findings clearly indicate a synergistic effect of waterpipe smoke, immobilization and darkness stress on serum ck levels, whereas serum alp levels of experimental groups have shown no significant synergism for the mentioned factors. results the results indicated enhanced serum ck and alp levels in all groups compared with control (p < 0.05). also, there was a significant differe...

three different experiments were conducted to study the effect of dietary astaxanthin on the immune response, resistance to white spot syndrome virus (wssv) and transcription of antioxidant enzyme genes in pacific white shrimp litopenaeus vannamei. each experiment included one triplicate treatment group (fed with 80 mg/kg astaxanthin supplemented diet) and one triplicate control group (fed with...

A sensitive, optimized assay for serum AMP deaminase (EC 3.5.4.6) is presented, which is based on a colorimetric indophenol determination of ammonia liberated during the enzyme reaction. An average enzyme activity in serum of 2.7 U/liter (n = 36, range 0-6.2) for healthy adults was established. Both the enzyme and colorimetric reactions were optimized to give an assay sensitivity of 0.2 U/liter...

An enzyme-linked immunosorbent assay (ELISA) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. A C3 and C11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (BSA). Polyclonal antibody against these two immunogens were prepared in New Zealand white rabbits. Purified Ig fractions of antibodies were immobilized o...