Incubation of mycelia of a virus-containing strain of Penicillium stoloniferum in a I % sodium dodecyl sulphate-4 % sodium chloride (SDS-NaCl) solution liberated protein and deoxyribonucleic acid within the first hours, whereas ribonucleic acid (RNA) release continued through an extended period. Although transfer RNA (tRNA) was readily solubilized by the treatment, viral doublestranded RNA (dsR...

Establishment of hydrophilic organic/salt-containing aqueous two-phase system and purification of salvianolic acid B from crude extract of S. miltiorrhiza by counter-current chromatography with said system were studied. Ethanol and n-propanol were selected to constitute biphasic systems with ammonia sulphate, sodium chloride and phosphate separately, and related system characteristics including...

Sesame and groundnut are important crops and a source of edible oil and protein. Amino acid content of both meals was analyzed and studies made on the protein solubility of defatted sesame and groundnut meal in aqueous solution over various pH and in various salt solutions. Valine, isoleucine, arginine, aspartic acid and glutamine were present in high concentration in both the defatted meals. A...

Selected alkaloids are chromatographed on cyanopropyl-silica thin layers using various nonaqueous and aqueous eluents. Because of the strong retention of these basic compounds, nonaqueous eluents containing medium polar diluents, strongly polar modifiers, and silanol blockers (ammonia or diethylamine) are required for separation. Likewise, aqueous eluents containing modifiers (acetonitrile, met...

Incubation of mycelia of a virus-containing strain of Penicillium stoloniferum in a I % sodium dodecyl sulphate-4 % sodium chloride (SDS-NaCl) solution liberated protein and deoxyribonucleic acid within the first hours, whereas ribonucleic acid (RNA) release continued through an extended period. Although transfer RNA (tRNA) was readily solubilized by the treatment, viral doublestranded RNA (dsR...

Polymer composites are among the most rapidly growing research fields in material science. Polymer composites are adding a great deal of material that is more durable and useful as compared to the conventional material. Properties of composite materials greatly depend on the nature, proportion and compatibility of the components of the composite materials. In this paper we have conducted an ana...

A thermally stable laccase was purified from the culture filtrate of Hexagonia tenuis MTCC-1119. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion-exchange chromatography on diethylaminoethyl (DEAE) cellulose. The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and native polyacrylamide gel electrophoresis (native-PAG...

Papain is a proteolytic enzyme of great commercial value. It cysteine protease highly expressed in Carica papaya fruit latex, but also present leaves. Purification procedures mostly deal with the latex and include combination precipitation and/or chromatographic techniques. Due to its solubility, structure activity characteristics, pH salt content play significant roles handling papain extracts...