Currently, there is a growing interest in the study of natural products, especially as part of drug discovery programs. Secondary metabolites can be extracted from a variety of natural sources, including plants, microbes, marine animals, insects, and amphibia. This chapter focuses principally on laboratory-scale processes of initial and bulk extraction of natural products from plant and microbi...

Reversed-phase high-performance liquid chromatography (RP-HPLC) of therapeutic proteins continues to play a significant role in product characterization. This study focuses on two key aspects of HPLC method development, namely the selection of organic modifier and the gradient shape. Separation of granulocyte colony-stimulating factor variants is being used as a case study to illustrate these c...

In this paper, the efficiency of lipid extraction from macrophytes (Chaetomorpha linum, Ulva laetevirens, Valonia aegagrophyla, Gracilariopsis longissima, Ruppia cirrhosa) for the biodiesel production is evaluated using four different methods based on the Accelerated Solvent Extraction technique. Both wet and dried tissues are extracted and effects induced on lipid recovery by the pressure and ...

Over the last four years, tremendous progress has occurred in field of organic photovoltaics (OPVs) and champion power conversion efficiency (PCE) under AM1.5G conditions, as certified by National Renewable Energy Laboratory (NREL), is currently 18.2%. However, these state-of-the-art devices were fabricated at lab-scale using highly toxic halogenated solvents which are harmful to human health e...

A predictive tool was developed to aid process design and rationally select optimal solvents for isolation of active pharmaceutical ingredients. The objective minimize the experimental work required a purification by (i) starting from selected crystallization solvent based on maximizing yield minimizing consumption (with constraint maintaining suspension density which allows crystal suspension)...