the aim of this study was to investigate the effect of adding different levels of zinc sulfate in semen extender on bovine sperm quality during cryopreservation. semen was collected from 4 holstein bull twice a week and the proper samples were pooled. semen were allocated to four extender treatments consisted of 0, 50, 100, 150 µm of zinc sulfate in 5 replicates and then, were frozen. post-thaw...

Dispersion of nuclear fibers of the spermatozoa of dogfish, man, and bull is made possible after treatment with a reducing and alkylating reagent coupled with an anionic detergent; the same detergent used at a low ionic strength dissociates the nuclear content of the rainbow trout sperm. Electron microscopy of such dispersed nuclear fibers has shown a beads-on-a-string configuration for these f...

Background: This study investigated the probable effects of daily administration of methamphetamine (MA) on sperm motility, acrosome reaction (AR), acridine orange (AO), sperm chromatin dispersion (SCD) test and chromomycin A3 (CMA3) in mice. Materials and Methods: Twenty four NMRI male mice were administrated three different doses of MA intraperitoneally (n=6 in each group). Different doses of...

Background: Following the expansion of human infertility, different procedures have been utilized for separation of normal sperm during ICSI (Intra cytoplasmic sperm injection). Hypo-osmotic swelling test (HOST) is one of the tests which have been introduced for assessment of integrity of sperm membrane when confronted with hypo osmotic condition. This test may also reflect intracellular organe...

Background: Vitamin E is believed to be the primary component of the antioxidant system in spermatozoa. There is a relationship between activity of these antioxidant and function of sperm. Vitamins E and C which are belong to non-enzymatic antioxidant are used as a supplemented drug to improve sperm quality and male fertility. The present study was carried out to investigate the positive effect...

BACKGROUND There is an association between aging ana an increased number of sperms with alterations in nuclear DNA. AIM To study the association between age and fragmentation of sperm DNA. MATERIAL AND METHODS Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was...

background: acrylamide (aa) is an important industrial chemical primarily. aa is also found in carbohydrate-rich foods that are prepared at high temperatures, such as french fries and potato chips. it is demonstrated that aa is a carcinogen and reproductive toxin and has ability to induce sperm damage. objective: the aim of this study was to observe the effects of aa on sperm parameters and eva...

Background: Because the etiopathogenesis of male infertility is multifactorial our study was designed to clarify relationship between standard semen parameters, testicular volume, levels reproductive hormones and fragmentation sperm nuclear DNA (SDF). Methods: Patients (n = 130) were clustered as subjects: 1) with an abnormal volum...