The presence of organic compounds and high amount of secondary metabolites (polysaccharides, phenolic component, etc.) in mosses cause difficulties in DNA extraction that are followed by problems in PCR reactions. In lower plants, various methods have been used for DNA extraction including silica gel and different commercial kits. These methods mostly use hazardous (like phenol or liquid nitrog...

A simple high-performance liquid chromatographic method with ultraviolet detection is proposed for the estimation of diclofenac potassium and drotaverine hydrochloride in human plasma. Liquid-liquid extraction was carried out with a mixture of dichloromethane-isopropyl alcohol (80:20, v/v). Chromatographic separation of the analytes and internal standard was achieved on an analytical 250 × 4.6 ...

abstract the increasing of yield per unit area is usually achievable by improving the soil fertility level through using (chemical) fertilizers. the purpose of the present research was to evaluate the 6 extraction methods including olsen, morgan, ammonium bicarbonate-dtpa, colwel, bray and mehlich 3 in order to specify a suitable extractant(s) for estimating available phosphorus through using 2...

  Background: Lavender’s essential oil is commonly used in aromatherapy and massage. Its major clinical benefits are on the central nervous system. Linalyl acetate and linalool are the most predominant chemical constituents in the essential oil of Lavandula angustifolia.   Objective: Comparison of super critical fluid extraction (SCFE) and hydrodistillation (HD) methods on Lavander΄s essential ...

Abstract: Background: Schizophrenia patients are given with a combined medication of Olanzapine (OLA) and Samidorphan (SAM). A sensitive bioanalytical RP-HPLC technique has to be needed analyse these drugs in biological samples, hence we have been developed method for simultaneous determination OLA SAM human plasma. Materials Methods: The separation was conducted on Zorbax SB-C18 column (250 mm...

The efficacy of melatonin (MLT) against ammonium acetate-induced neurotoxicity was biochemically studied in the experimental rats. The activities of serum transaminases and the levels of thiobarbituric acid reactive substances were significantly increased in ammonium acetate-treated rats. These levels were significantly decreased in MLT and ammonium acetate-treated rats. Further, non-enzymatic ...

the volatile components of citrus aurantium l. flowers were characterized by gc-ms with two different extraction techniques, hydrodistillation (hd) and ultrasonic-assisted headspace solid phase microextraction (ua-hs-spme). in the spme method, the volatile components of the samples, irradiated by ultrasonic radiation, were collected on a polydimethyl siloxane (pdms) commercial fiber as well as ...

A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solidphase extraction. The method was performed on a Luna C 18 column and the mobile phase consisted of acetonitrile:water (95:5, v/ v)/ammonium acetate (p...