There is a very limited number of existing probes whose fluorescence is turned on in the presence of the class of biological thiols made up of glutathione, cysteine, and homocysteine. The extant probes for this class of biological thiols commonly have poor aqueous solubility and long analyte response times, and they demand a very high probe/thiol ratio for decreased time of significant reporter...

Keywords: endothelial dysfunction, NO, nitrosative stress, reduced thiols, S-derivatives of 1,2,4-triazole

A large number of proteins contain free thiols that can be modified by the formation of internal disulphides or by mixed disulphides with low-molecular-mass thiols. The majority of these latter modifications result from the interaction of protein thiols with the endogenous glutathione pool. Protein glutathionylation and disulphide formation are of significance both for defence against oxidative...

Eight novel compounds were prepared by reaction of 5-(bromo- propoxyphenyl)-10,15,20-triphenylporphyrin with oxazole thiols, 1,3,4-oxadiazole thiols and 1,3,4-thiadiazole thiols, and their structures confirmed by UV-vis, IR, 1H-NMR, MS and elemental analysis. The assessment of indirectly measured 1O(2) production rates against 5,10,15,20-tetraphenyl porphyrin (H(2)TPP) were described and the re...

For the first time, synthesis of thioesters starting from enals and thiols has been performed in presence a bulky N-heterocyclic carbene (NHC) as catalyst.

Serum copper, ceruloplasmin, protein thiols and thiobarbituric acid reactive substances (TEARS) were estimated in 25 patients of liver cancer. The copper to ceruloplasmin ratio was moderately increased (P<0.05) but the copper (P<0.001) and ceruloplasmin (P<0.001) levels were significantly increased in liver cancer patients when compared to controls. Protein thiols levels were found to be highly...

An effective phosphine-catalyzed method has been developed for the enantioselective addition of aryl thiols to the γ position of allenoates, thereby furnishing ready access to aryl alkyl sulfides in very good ee. An array of mechanistic data are consistent with addition of the chiral phosphine to the allenoate being the turnover-limiting step of the catalytic cycle. The optimized reaction condi...

Free sulfhydryl and disulfide groups of an acid-soluble and acid-precipitable fraction and of isolated plasma membranes were quantified in fresh and stored human platelets. Fresh platelets contained 26.6 X 10 moles of total thiols including hee sulfhydryl and disulfide groups per iO cells. One-third of these thiols were present as disulfides. Acid-soluble thiols accounted for one-tenth of the t...

Free sulfhydryl and disulfide groups of an acid-soluble and acid-precipitable fraction and of isolated plasma membranes were quantified in fresh and stored human platelets. Fresh platelets contained 26.6 X 10 moles of total thiols including hee sulfhydryl and disulfide groups per iO cells. One-third of these thiols were present as disulfides. Acid-soluble thiols accounted for one-tenth of the t...