Morphological examination of spermatozoa remains a valuable tool for determining the quality of feline sperm for cryopreservation and/or for use in artificial insemination. Numerous staining techniques have been employed for feline sperm assessment, and several variations have also been reported. Forty-eight domestic cats were castrated and epididymal sperm samples were obtained. Sperm morpholo...

Specimens from 495 patients attending Johannesburg hospitals and family planning clinics were examined for Trichomonas vaginalis by microscopy of Giemsa (GS), Papanicolaou (PAP), and acridine-orange (AO) stained smears, and by culture in Feinberg-Whittington medium. Culture, Pap and GS stained smears from vaginal swabs yielded fewer positives than AO stained smears. Although Pap-stained cytolog...

objective: trichomonas species usually reside in the mouth and occasionally in the respiratory tract. these species can be found in the lungs of humans. although the pathogenicity of the parasite in the respiratory system has not been proven, it is more prevalent in people who lack good oral health or suffer from asthma or chronic pulmonary diseases. in the present descriptive study, we have id...

OBJECTIVES The main objective of this study was to assess antral brush cytology as an alternate approach for the diagnosis of H. pylori infection as compared to histopathology. METHODS 75 cases with diagnosis of antral gastritis underwent endoscopic antral biopsy as well as antral brushing cytology was included irrespective of age and sex. Cytological slides were stained with Giemsa stain & b...

BACKGROUND AND OBJECTIVES To determine the distribution of embryonic and fetal hemoglobin chains in fetal erythroblasts isolated from maternal blood in the first trimester of pregnancy and establish the feasibility of using these chains as markers for fetal cell identification. DESIGN AND METHODS Maternal blood was obtained from 187 singleton pregnancies at 11-14 weeks of gestation immediatel...

-This paper proposes an improved two-step classification scheme for Giemsa-stained human chromosomes using a hierarchical multilayer neural network with an error back-propagation training algorithm. In the first step, the Group Classifier (GC), a two-layer neural network, classifies chromosomes into seven groups based on their morphological features, such as relative length, relative area, and ...

on a conventional ultramicrotome. Semi-thin sections on grids were stained with the Giemsa method and methylene blue-azure II-basic fuchsin. Staining without removal of the embedding matrix was sharp and brilliant. These stained sections were observed under a light microscope. For electron microscopy, they were doubly stained with uranyl acetate and lead salt. Identical sites on such sections c...

We carried out this work with the purpose of studying the effects of protein and vitamin B deficiency on the morphologic and quantitative aspects of the myenteric plexus of the descending colon of adult Rattus norvegicus. Twenty-eight rats were divided in two groups, one of them receiving chow with 22% protein level (control) and the other fed with chow having 8% protein level without vitamin B...