plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...

agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. indeed, upon infection of eukaryotic cells, agrobacterium tumefaciens transfers a piece of its tumor inducing (ti)-plasmid, called t-dna, to the host cell nucleus, which subsequently integrates into the host genome. the vird2 virulence protein which has relaxase endonuclease activities covalently binds ...

two different growth media, namely complex and defined media, were used to examine establishment of steady-state conditions in phosphate-limited culture system of saccharomyces cerevisiae cen.pk113-5d strain. using the defined growth medium, it was possible to obtain steady state condition in the continuous culture. the effect of phosphate concentration on the growth of s. cerevisiae in phospha...

Spent brewer’s yeast is a by-product rich in components with biological activity, being considered an innovative and economical viable solution for creating sustainable functional food systems. This work describes the use of ultrasound treatment, performed under various conditions, aim to facilitate cell lysis further characterize released cellular content spent yeast. Solid residue suspensions...

objective(s):some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (mscs). however, the interaction between mesenchymal stem cells and macrophage is not clear yet.  the current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived mscs and rat macrophages. materials...

The armadillo could be considered as an experimental model for leprosy studies. Mycobacterium leprae main host cells are macrophages and Schwann cells. However, endocytosis and germicidal activities of armadillo macrophages and monocytes have not yet been evaluated. The aim of this research was to evaluate endocytosis and NBT-reduction activities of monocyte-derived macrophages (MDM) and monocy...

To the best of our knowledge, there are no studies on effects molecular hydrogen (H2) microorganisms. In this study, we performed co-culture experiments using two microorganisms involved in sake brewing: yeast strain K1401 and kuratsuki bacterium Kocuria TGY1127_2. The cells were suspended water or containing H2 statically incubated at 4 °C for 2 h before co-culture. Sake taste was estimated a ...

Glutinous rice tape circulating in the community generally does not contain probiotic cells. However, adding Lactobacillus plantarum Dad-13 and Saccharomyces boulardii can increase its potential as a food. This study is aimed to determine effect of using glutinous varieties addition cells on number lactic acid bacteria (LAB) yeast, levels anthocyanins, antioxidant activity, well preference leve...

Super-resolution microscopy includes multiple techniques in optical that enable sub-diffraction resolution fluorescence imaging of cellular structures. Expansion (EXM) is a method physical expansion to obtain super-resolution images biological sample on conventional microscopy. We present yeast organelles, applying the combination and ExM techniques. When preparing pre-expanded samples, methods...