Thermal stability of the thermolysin-like protease produced by Bacillus stearothermophilus (TLP-ste) is highly dependent on calcium at concentrations in the millimolar range. We describe the rational design and production of a fully active TLP-ste variant whose stability is only slightly dependent on calcium concentration.

This study was designed to quantitatively analyze the expression of thaumatin-like protein (TLP) at the transcriptional level in different sorghum lines when they were infested with greenbugs. Three sorghum lines, Tx7000, PI550607, and PI550610, were used. RNAs were isolated from the different sorghum lines that were infested with greenbugs at different infestation times. The resultant mRNA was...

Currently, floating wind turbines (FWTs) may be the more economical and suitable systems with which to exploit offshore wind energy in deep waters. Among the various types of floating foundations for offshore wind farms, a tension leg platform (TLP) foundation can provide a relatively stable platform for currently available offshore wind turbines without requiring major modifications. In this s...

To investigate the genomic architecture underlying the quintessential adaptive phenotype, antifreeze glycoprotein (AFGP) that enables Antarctic notothenioid survival in the frigid Southern Ocean, we isolated the AFGP genomic locus from a bacterial artificial chromosome library for Dissostichus mawsoni. Through extensive shotgun sequencing of pertinent clones and sequence assembly verifications,...

Transient lunar phenomena (TLPs) have been reported for centuries, but their nature is largely unsettled, and even their existence as a coherent phenomenon is still controversial. Nonetheless, a review of TLP data shows regularities in the observations; a key question is whether this structure is imposed by human observer effects, terrestrial atmospheric effects or processes tied to the lunar s...

As systems on chip are evolving to networks on chip (NOC) providing a unified communication infrastructure for a number of computational resources, being able to easily implement computational tasks as a parallel program that can be efficiently executed by multiple resources together is becoming increasingly important. Recent advances in thread-level parallel (TLP) architectures have made it po...