UNLABELLED Bacterial genomes encode numerous homologs of Cas9, the effector protein of the type II CRISPR-Cas systems. The homology region includes the arginine-rich helix and the HNH nuclease domain that is inserted into the RuvC-like nuclease domain. These genes, however, are not linked to cas genes or CRISPR. Here, we show that Cas9 homologs represent a distinct group of nonautonomous transp...

Software components provide essential means to structure and organize software effectively. However, frequently, required component abstractions are not available in a programming language or system, or are not adequately combinable with each other. Invasive software composition (ISC) is a general approach to software composition that unifies component-like abstractions such as templates, aspec...

The effect of lipopolysaccharide (LPS) from Klebsiella pneumoniae on the bioelectric properties of canine cultured tracheal epithelium was examined. LPS decreased short-circuit current (Isc), and its effects on Isc were reduced when Isc was inhibited by amiloride and indomethacin. We speculate that LPS may selectively inhibit Na+ absorption through the inhibition of prostaglandin synthesis by a...

The effect of lipopolysaccharide (LPS) from Klebsiella pneumoniae on the bioelectric properties of canine cultured tracheal epithelium was examined. LPS decreased short-circuit current (Isc), and its effects on Isc were reduced when Isc was inhibited by amiloride and indomethacin. We speculate that LPS may selectively inhibit Na+ absorption through the inhibition of prostaglandin synthesis by a...

For 7H-furo[3,2-g][1]benzopyran-7-one (psoralen), intersystem crossing (ISC) rate constants have been computed. Employing the Fermi golden rule, the harmonic approximation, and a pure-spin Born-Oppenheimer basis, both direct and vibronic spin-orbit (SO) coupling has been taken into account. Necessary data on electronic excitation energies and potential energy hypersurfaces originate from correl...

Delayed hypothermia reduces ischemic hippocampal CA1 injury. However, there are residual structural and functional abnormalities. Therefore, we studied whether these apparently vulnerable rescued neurons are susceptible to secondary insults. All gerbils were subjected to normothermic forebrain ischemia (ISC, 5 min) or SHAM operation. Gerbils were treated with mild hypothermia (HYPO; 33 degrees ...

This qualitative study explores perspectives of U.S.A.-based science communication researchers and practitioners who attended a symposium focused on advancing inclusive (ISC). ISC is growing global movement that aims to center equity, inclusion, marginalized in communication. Findings underscore the complexity systemic barriers ISC, critical need for resource sharing network building, importanc...

Strength loss following disuse may result from alterations in muscle and/or neurological properties. In this paper, we report our findings on human plantar flexor muscle properties following 4 wk of limb suspension (unilateral lower limb suspension), along with the effect of applied ischemia (Isc) on these properties. In the companion paper (Part II), we report our findings on the changes in ne...