Low-Density Lipoprotein (LDL) extracted from egg yolk has recently known can eliminate the adverse effect associated in use of fresh yolk. The role LDL liquid preservation at 4°C ram sperm not been explored. This research evaluates effects substituting with 4 °C on 5 days. objective this was to assess for as an extender 4°C, well spermatozoa motility, viability, morphology, plasma membrane, and...

The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined a...

The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spermatozoa, suggesting that in equine sperm cryopreservation protocols, the addi...

In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...

It is known that livestock animal semen very sensitive to cold shock during freezing processes, and this sensitivity directly affects post-thaw progressive motility, mitochondrial membrane potential, sperm nuclear DNA integrity in vitro spermatological parameters such as plasma acrosome integrity, fertility. In addition, with the sudden decrease total antioxidant level of after thawing, cells a...

Abstract The study was executed to investigate the different cooling periods (0, 24, and 48 hours) effects on plasma membrane integrity depending hypo osmotic swelling test (HOST), spermatozoa motility, acrosome integrity, live sperm, abnormalities, correlation among Host other rams sperm parameters This done at an animal farm in Al-Kut city (southern Baghdad city\ Iraq), from 5th January Febru...

objective: the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. materials and methods: in this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in bioxcell ® and tris-citric...

The aim of the present study was to evaluate cryoprotective effect trehalose on stallion semen quality like progressive sperm motility, viability, acrosome integrity, membrane integrity. A total 42 ejaculates from six Marwari (seven ejaculate each) were used. Immediately after gross evaluation, filtered into a pre warm, graduated measuring bottle get gel free and it diluted with primary extende...

Background Cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been described for activation of a temporary response in oocyt...