Mycotoxin is a worldwide problem threatening animal health and performance as well public health. The objective of this experiment was to test the effect ammonization on elimination common mycotoxins in laying hen compound feed (CF) dairy cattle total mixed ration (TMR). CF for hens TMR cows were contaminated with commonly occurring [aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZEA)] a...

To assess the incidence of aflatoxins contamination in different commodities, a representative countrywide study was conducted. One hundred composed samples of cereals, grains, spices, condiments and refined and raw sugars were analysed for aflatoxin B1, B2, G1 and G2. The moisture contents of all the food samples were also invariably determined. Only 6% of the samples namely maize and red chil...

the aim of the present work was to determine whether the administration of melatonin or l­-tryptophan (a precursor of melatonin) affects the immune responses and performance of broilers during induced exposure to aflatoxins in feed. the study was conducted from 0-6 weeks comprising six dietary treatments in triplicate with10 chickens in each replicate. the diets were formulated to supply 23% cr...

A system for the formation of an aflatoxin B1-reduced glutathione conjugate in vitro was developed, capable of yielding 80% conversion of aflatoxin B1 into the conjugate. A reverse-phase high-pressure-liquid-chromatography system was also devised that not only facilitates improved resolution of the compound but that, by manipulation of the pH, is also capable of an extensive purification of the...

The present study was conducted to determine whether or not aflatoxin B1 was an effective inducing agent for lysogenic bacteria and to characterize some of the parameters involved in induction. A lysogenic strain of Bacillus megaterium (NRRL-B-3695) and an indicator strain of this species (NRRL-B-3694) were used. Cultures of the lysogenic strain were incubated for various periods of time in the...

This paper describes the effect of temperature on the level of aflatoxin production in Mucuna pruriens seeds. The highest level of aflatoxin B1 (1.75 micrograms/g) was detected in the samples incubated at 25 degrees C for three weeks. At 20, 30, and 35 degrees C, aflatoxin levels were 0.30 to 0.56, 0.37 to 1.20, and 0.26 to 0.65 micrograms/g, respectively. The lowest concentration of aflatoxin ...

The physiological effects of DL-ethionine and related methionine analogs upon cellular protein synthesis and methyl group transfer to aflatoxin B1 in Aspergillus parasiticus were examined in growing and resting cell systems. The addition of DL-ethionine (0.02 M) to growing mycelia inhibits cellular growth some 37% after 120 h of development. Addition of ethionine before 50 h prevents any aflato...

Twelve forms of human hepatic cytochrome P450 were expressed in hepatoma cells by means of recombinant vaccinia viruses. The expressed P450s were analyzed for their abilities to activate the potent hepatocarcinogen aflatoxin B1 to metabolites having mutagenic or DNA-binding properties. Five forms, P450s IA2, IIA3, IIB7, IIIA3, and IIIA4, activated aflatoxin B1 to mutagenic metabolites as assess...