1. Isoelectric focusing of human liver alkaline phosphatase in a sucrose density gradient with LKB Ampholine as carrier ampholytes is described. 2. Problems due to the chelating properties of the ampholytes and the pH gradient were examined. 3. A reactivation procedure to counter these effects was devised that can probably be used for other alkaline phosphatases. 4. The isoelectric point of hum...

cells derived from primary tumors were smeared or sec tioned and stained histochemically for alkaline phosphatase by use of a simultaneous azodye coupling technique with or without amino acid inhibitors (Dand i.-phenylalanine and i.-homoarginine) in the incubation mixtures. These amino acids are able to distinguish the Regan isoenzyme from nonRegan alkaline phosphatases. Kaplow's scoring technique

Autotrophic organisms obtain phosphorus from the environment by secreting alkaline phosphatases that act on esters, resulting in inorganic phosphate that is then taken up. New work shows that the cyanobacterium Aphanizomenon ovalisporum obtains inorganic phosphate by secreting the cyanotoxin cylindrospermopsin, which induces alkaline phosphatase in other phytoplankton species.

Alkaline phosphatase enzymes are found throughout the living world and fulfil a variety of functions. They have been linked to regeneration, stem cells and biomineralisation in a range of animals. Here we describe the pattern of alkaline phosphatase activity in a spiralian appendage, the operculum of the serpulid polychaete Pomatoceros lamarckii. The P. lamarckii operculum is reinforced by a ca...

* I HE distribution of phosphatases during the embryonic development of X Drosophila melanogaster Meig. was chosen as the next object of study for three main reasons: (i) a satisfactory technique for the demonstration of phosphatase (at least in the case of alkaline phosphatase) is available-, (2) the possible significance of phosphatases during embryogenesis is indicated by the work of Moog (1...

A patient with hypophosphatasia and her relatives, including an affected brother, were studied with regard to serum alkaline phosphatase levels, phosphoethanolamine excretion, leucocyte alkaline phosphatase, blood group genes, and alkaline phosphatase distribution in the serum protein fractions. The pattern of alkaline phosphatase distribution in the serum protein fractions was normal both in t...

In the present study, we identified protein phosphatases dephosphorylating centrins previously phosphorylated by protein kinase CK2. The following phosphatases known to be present in the retina were tested: PP1, PP2A, PP2B, PP2C, PP5, and alkaline phosphatase. PP2C α and β were capable of dephosphorylating P-Thr(138)-centrin1 most efficiently. PP2Cδ was inactive and the other retinal phosphatas...

In the present study, we identified protein phosphatases dephosphorylating centrins previously phosphorylated by protein kinase CK2. The following phosphatases known to be present in the retina were tested: PP1, PP2A, PP2B, PP2C, PP5, and alkaline phosphatase. PP2C α and β were capable of dephosphorylating P-Thr-centrin1 most efficiently. PP2Cδ was inactive and the other retinal phosphatases al...

The three human isozymes of alkaline phosphatases were quantitatively determined in normal testis and seminoma tissues. The highly selective assays were based on isozyme specific monoclonal antibodies. In the normal testis approximately 90% of the catalytic activity originates from the tissue unspecific alkaline phosphatase, and the remaining activity was due to trace expression of both intesti...