نتایج جستجو برای: bacmid shuttle vector

تعداد نتایج: 203458  

2007
Bruce Kirkpatrick Magalie Guilhabert A. H. Purcell Andy Walker

INTRODUCTION One of our projects involved the development of a transformation and transposon mutagenesis systems for the bacterium that causes Pierce's disease (PD), Xylella fastidiosa (Xf). We now have a random transposon mutagenesis system working for Xf (Guilhabert, et al. 2001) and recently we have developed an E. coli/Xf plasmid shuttle vector. We are currently assessing the stability of t...

Journal: :Applied and environmental microbiology 1989
A Y Kim H P Blaschek

A stable shuttle vector which replicates in Escherichia coli and Clostridium perfringens was constructed by ligating a 3.6-kilobase (kb) fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 kb). The marker for this shuttle plasmid originated from the 1.3-kb chloramphenicol resistance gene of plasmid pHR106. The resulting shuttle vector, designated pAK201, is 8 kb in size and codes...

Journal: :Virus research 2003
Hanzhong Wang Fei Deng Gorben P Pijlman Xinwen Chen Xiulian Sun Just M Vlak Zhihong Hu

Purification of genotypes from baculovirus isolates provides understanding of the diversity of baculoviruses and may lead to the development of better pesticides. Here, we report the cloning of different genotypes from an isolate of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV) by using a bacterial artificial chromosome (BAC). A transfer vector (pHZB10) was constructed ...

2016
Manoochehr Makvandi Ali Teimoori Niloofar Neisi Alireza Samarbafzadeh

BACKGROUND The hepatitis E virus (HEV) accounts for hepatitis E infection with relatively high mortality rate in pregnant women that can lead to fulminant hepatitis. The baculovirus expression system (BES) has the capability to produce high-level recombinant proteins and could be useful for vaccine designing. OBJECTIVES The aim of this study was designing a recombinant hepatitis E virus ORF2 ...

2013
Donghui Song Jing Li Xiaoxu Hu Bo Xi

The construction of an integrative shuttle expression vector and potential utility was reported in Escherichia coli and Anabaena (Nostoc) sp. strain PCC 7120. The vector comprised of the following elements: (a) an intergenic non-coding region from Anabaena to facilitate its genomic integration (b) a strong functional PpsbAI promoter from Anabaena for desired gene expression and (c) neomycin pho...

Journal: :Journal of virology 2008
Oihane Simón Trevor Williams Aaron C Asensio Sarhay Ros Andrea Gaya Primitivo Caballero Robert D Possee

The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid (Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies (OBs) produced by the Sf29null bacmid in comparison to viruses containing this gen...

Journal: :Nucleic Acids Research 2005
Richard Pelletier Brian T. Farrell Juan José Miret Robert S. Lahue

Trinucleotide repeats (TNRs) undergo high frequency mutagenesis to cause at least 15 neurodegenerative diseases. To understand better the molecular mechanisms of TNR instability in cultured cells, a new genetic assay was created using a shuttle vector. The shuttle vector contains a promoter-TNR-reporter gene construct whose expression is dependent on TNR length. The vector harbors the SV40 ori ...

2015
Xiao-lin Zhang An-min Jiang Zhong-you Ma You-yi Xiong Jin-feng Dou Mei-song Qin Jian-fei Wang

This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori. The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus expression system. To determine whethe...

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