Analytical cytometry has significant potential beyond cellular analysis. The inherent capability of flow cytometers to efficiently discriminate between uniformly sized particles based on their intrinsic properties provides the foundation for multiplex bead assays. The technology can be exploited in designing immunoassays, Western blot-like antibody assays, and nucleic acid hybridization assays....

The one-trial passive avoidance learning task is commonly used in avian research to explore anatomical, cellular and molecular parameters of learning and memory. Many factors are known to influence the effectiveness and/or duration of such learning events. Combinations of novel odours, such as pyrazine, and aposematic colours, such as brig ht yellow or red, have been shown to induce a long-last...

Small-angle x-ray solution scattering (SAXS) is analyzed with a new method to retrieve convergent model structures that fit the scattering profiles. An arbitrary hexagonal packing of several hundred beads containing the problem object is defined. Instead of attempting to compute the Debye formula for all of the possible mass distributions, a genetic algorithm is employed that efficiently search...

Herein, we investigated the wetting behavior of hexagonally close-packed polystyrene bead arrays with different bead diameters and surface flatness. The contact angle was found to be influenced by the surface roughness as well as the contact area of the polystyrene bead array with a water droplet.

We show how the trapping position of an optically trapped 1-µm polystyrene bead is measured, and that it can be shifted by changing the relative radiation pressure exerted on the bead. The trapping position of the bead relative to the focus is measured using defocusing microscopy and 2-photon microscopy. Our observations are explained by the two component approach for optical forces.

BACKGROUND A novel, bead-based flow cytometric assay was developed for simultaneous determination of antibody responses against Toxoplasma gondii and Trichinella spiralis in pig serum. This high throughput screening assay could be an alternative for well known indirect tests like ELISA. One of the advantages of a bead-based assay over ELISA is the possibility to determine multiple specific anti...

BACKGROUND Direct sputum smear microscopy is the mainstay of TB diagnosis in most low and middle income countries, and is highly specific for Mycobacterium tuberculosis in such settings. However it is limited by low sensitivity, particularly in HIV co-infected patients. Concentration by centrifugation has been reported to be more sensitive than direct smear preparation, but is only suitable for...

This paper presents work on the development of a microfluidic device using super-paramagnetic beads for sampling and mixing. The beads are manipulated via an external rotating permanent magnet in a microfluidic channel. Efficient mixing is achieved in a short distance with this method. Modeling shows the variables which influence the mixing are flow rate, bead rotation speed and the bead number...