Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

Abstract Mulberry ( Morus alba L.) leaf, a “source of both medicine and food”, contains antioxidant ingredients such as flavonoids, alkaloids polyphenols. The effects 6-benzylaminopurine (6-BA) treatment on plant growth flavonoid contents in mulberry leaves were investigated this study. expression rutin (Rut), chlorogenic acid (ChA), isoquercitrin (IQ) astragaloside IV (Ast) related genes the s...

ABSTRACT Gerbera (Gerbera jamesonii Bolus ex Hook. F.; Asteraceae), is one of the most economically important ornamental plants due to its aesthetic value. In present study, we established a micropropagation method for large-scale production quality planting material gerbera ‘Pink Melody’. Eighty-six percent capitulum explants produced adventitious shoots (15.44 ± 0.34 per capitulum) on Murashi...

Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6BA) hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 μM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrati...

An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneratio...

Abstract Orchids with their sheer variety of species are amazing, a major sharer in global floriculture trade. Papilionanthe Miss Joaquim, well known hybrid orchid, also recognized as official National flower Singapore is resilient, sun loving which blossoms throughout the year. Though seed pod derived from single contains millions dusty seeds, due to lack endosperm, seeds cannot germinate with...