«binding parameters»
۱۳٬۳۸۱ نتیجهStructure-function relationships in the four repeats of human interphotoreceptor retinoid-binding protein (IRBP).
PURPOSE Interphotoreceptor retinoid-binding protein (IRBP) binds hydrophobic ligands in the interphotoreceptor space. Human IRBP consists of 1230 amino acids in four 300 amino acid long repeats. We asked: 1. Whether each of the four repeats can bind retinoids or fatty acids, 2. W
Insights into T Cell Recognition of Antigen: Significance of Two-Dimensional Kinetic Parameters
The T cell receptor (TCR) interacts with peptide-major histocompatibility complex (pMHC) to enable T cell development and trigger adaptive immune responses. For this reason, TCR:pMHC interactions have been intensely studied for over two decades. However, the details of how variou
Development of increased cytoplasmic binding of androgen in the submandibular gland of the mouse with testicular feminization.
The binding of [l ,2-3H]dihydrotestosterone by cytoplasmic extracts of submandibular glands from normal male mice and from mice carrying the X-linked gene for testicular feminization (Tfm/Y) has been studied by density gradient centrifugation and gel filtration. Androgen binding
Vagus nerve modulates secretin binding sites in the rat forestomach.
Secretin is well known for its inhibitory action on gastric motility. It has been reported that secretin in a physiological dose inhibits gastric motility through mediation by the vagal afferent pathway. Secretin also elicited relaxation of carbachol-stimulated rat forestomach mu
Amphibians as a model to study endocrine disruptors: I. Environmental pollution and estrogen receptor binding.
Many chemicals released into the environment without toxicological risks have the capacities to disrupt the function of endocrine systems. These endocrine disruptors disturb normal endocrine mechanisms and have been observed in nearly all classes of vertebrates. The aim of this r
Pharmacological distinction between dantrolene and ryanodine binding sites: evidence from normal and malignant hyperthermia-susceptible porcine skeletal muscle.
Dantrolene inhibits and ryanodine stimulates calcium release from skeletal-muscle sarcoplasmic reticulum (SR), the former by an unknown mechanism, and the latter by activating the ryanodine receptor (RyR), the primary Ca2+-release channel of SR. Dantrolene is used to treat malign
Novel opioid binding sites associated with nuclei of NG108-15 neurohybrid cells.
Nuclear opioid binding sites have been discovered in NG108-15 neurohybrid cells. Marker enzyme analyses as well as electron and fluorescence microscopy studies attested to the high degree of purity of the nuclear preparations. Immunohistochemical studies on cryostat sections of N
Binding of 3H-estradiol-17 beta-(beta-D-glucuronide), a cholestatic organic anion, to rat liver plasma membranes. Evidence consonant with identification of organic anion carriers.
The binding of 3H-estradiol-17 beta(beta-D-glucuronide) (3H-E2 17G), a cholestatic organic anion, was examined in rat liver plasma membranes, and two saturable, specific binding sites were identified. The binding parameters are Kd1 = 3.9 X 10(-7) M, Bmax1 = 69 pmol/mg of protein;
Cation binding and conformational changes in VILIP and NCS-1, two neuron-specific calcium-binding proteins.
VILIP and NCS-1, neural-specific, 22-kDa Ca(2+)-binding proteins possessing four EF-hands, were expressed in Escherichia coli to study their divalent cation properties. Flow dialysis (Ca2+ binding) and equilibrium gel filtration (Mg2+ binding) revealed that both recombinant prote
Carcinogen-binding proteins in the rat ventral prostate: specific and nonspecific high-affinity binding sites for benzo(a)pyrene, 3-methylcholanthrene, and 2,3,7,8-tetrachlorodibenzo-p-dioxin.
The polychlorinated dibenzodioxin [3H]-2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) and the carcinogens [3H]benzo(a)pyrene and [3H]-3-methylcholanthrene bound to saturable binding sites in cytosol from the rat ventral prostate. Analysis of equilibrium binding parameters in diluted