Whitehead (1965) recently showed that a measurement of the amount of hydroxyproline excreted in the urine was of value in detecting marginal malnutrition. He also suggested that a simultaneous measurement of the plasma amino acid ratio (Whitehead, 1964) provided a biochemical means of differentiating between malnutrition, in which the primary deficiency was one of protein, and undernutrition du...

Rapid biochemical tests for nitrate, indole, gelatin, starch, esculin, and o-nitrophenyl-beta-D-galactopyranoside were performed on 112 strains of anaerobic bacteria. All tests were incubated under aerobic conditions, and results were recorded within 4 h. The tests for nitrate, indole, and starch showed a 95% or greater correlation when compared to the standard biochemical tests. Tests for escu...

results the pcr approved the bacillus genus of the isolates. the strain of bacillus subtilis was identified using biochemical tests. 40 ºc and ph 11 are the optimum condition for maximum keratinase enzyme activity. objectives the aim of this study was the isolation of feather degrading bacillus spp.from a poultry waste and the optimization of conditions for the highest enzyme activity and feath...

There are many methods to identify anaerobic nonsporeforming bacilli: histological, bacteriological (biochemical test, microsystem API 20 A), serological, cell wall composition analysis, molecular methods and gas-liquid chromatography (GLC). A comparison between biochemical tests and gas-liquid chromatography was made in this study for the identification of this group of microorganisms. GLC con...

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the ...

Markers of xenobiotic metabolization (cytochrome P450, ethoxyresorufin-O-deethylase, glutathione and glutathione-S-transferase) were investigated in the liver of the common carp Cyprinus carpio after 28-day exposure to different pesticide formulations.The fish exposed to herbicide Sencor 70 WG (metribuzin 700 g/kg) of 0.25 and 2.5 mg/l showed no change in cytochrome P450 and activity of ethoxyr...