X-ray pulses (full width at half maximum ∼ 90 ns, dose rate 107 Gy s−1) were used to irradiate the monolayer of peripheral blood mononucleated cells using PF-2kJ kilojoule plasma focus device. Four different exposure conditions evaluated 5, 10, 20, and 40 pulses, with mean measured by TLD-100 being 0.12 ± 0.02 mGy, 0.14 0.03 0.22 0.06 0.47 0.09 respectively. Cytogenetic analysis showed an incre...

As two cytogenetic parameters of radiation exposure, the frequency of micronucleus in erythroblasts, lymphocytes and red cells (Howell-Jolly body) as well as chromosome aberrations in bone marrow cells and in lymphocytes were studied in 24 thorotrast patients and in 32 atomic bomb (A-bomb) survivors who were exposed within one kilometer from the Hiroshima hypocenter. The incidence of both micro...

Background: Rapidly increasing possibilities of exposure to environmental extremely low-frequency electromagnetic fields (ELF-EMF) have become a topic of worldwide investigation. Epidemiological and laboratory studies suggest that exposure to ELF-EMF may increase cancer risk therefore assessment of chromosomal damage in various cell lines might be of predictive value for future risk es...

Background: Constitutional chromosome abnormalities are among the major contributors to the genetic causes of reproductive disorders. Despite all of worldwide efforts have been made so far, the prognosis for mosaic X chromosome aberration below 30% of unemployed has yet to be established. The purpose of this study was to assess the quantity and quiddity of chromosomal aberrations that may negat...

PURPOSE To validate whether the number of aberrations could be used as a measure of the radiosensitivity of human tumour cells. If so, this would potentially provide a more rapid method than the colony assay to predict radiocurability in human tumour biopsy material. MATERIALS AND METHODS A panel of 13 human tumour cell lines was investigated, covering a wide range of radiosensitivities. Fluo...

The effects of mitomycin C (MMC) and ethyl methanesulfonate (EMS) on the frequency of structural and numerical chromosomal aberrations were evaluated in bovine peripheral lymphocytes in vitro using fluorescence in situ hybridization (FISH) technique. Three whole chromosome painting probes (WCPs), BTA 1 for the bovine chromosome 1 (red colour), BTA 5 for chromosome 5 (green colour) and BTA 7 for...

Chromosomal aberrations and dihydrofolate reductase gene amplification are observed in L5178Y mouse lymphoma cells after treatment with hydroxyurea. The types of aberrations include polyploidy, endoreduplication, chromosome fragmentation, and the presence of extrachromosomal DNA. Hydroxyurea-treated cells analyzed by cell sorting showed a subpopulation of cells with increased DNA and increased ...

Numerical chromosome aberrations by interphase cytogenetic analysis have been reported in a few samples of colorectal neoplasms. No studies have defined a distinct relationship between these aberrations and clinicopathological features. To investigate the chromosome aberrations as a marker of invasiveness or prognosis, we conducted an interphase cytogenetic study using fluorescence in situ hybr...