A multiple-primer PCR was used to identify genes encoding aminoglycoside-modifying enzymes in 381 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). The technique used three sets of primers delineating specific DNA fragments of the aph(3')-III, ant(4')-I, and aac(6')-aph(2") genes, which influence the MICs of gentamicin, tobramycin, and lividomycin. Isolates with none of t...

Catheter related bloodstream infections are a major problem in most tertiary care hospitals. Among the various organisms associated with nosocomial infections, coagulase negative staphylococci are responsible for majority of the catheter related infections. They are usually resistant to standard antibiotics necessitating prolonged hospital stay and amplifying the cost of treatment manifold whic...

The intrinsic resistance of methicillin-resistant Staphylococcus aureus (MRSA) is frequently explained by the production of an additional penicillin-binding protein (PBP), which is encoded by the mecA gene. The mec regulator genes, mecR1 and mecI, was identified in mecA-carrying Staphylococcus aureus N315. Between February and March, 1993, 179 clinical MRSA isolates were collected from institut...

Aerobic In clusters ● Coagulase (+): Staphylococcus aureus ● Coagulase (-): Staphylococcus lugdunensis and other coagulase-negative staphylococci In pairs/chains

The objective of this study was to identify bacteria causing gangrenous ovine mastitis as well as to detect and genotype Staphylococcus aureus in milk samples by using polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP) of coagulase gene. A total of 264 milk samples were collected from Awassi sheep with gangrenous mastitis raised in Sanliurfa provin...

A total of 272 staphylococcal isolates from cases of bovine mastitis (159 Staphylococcus aureus) belonging to 12 different species were identified with ID32 STAPH galleries, and 51 of them were confirmed by 16S rRNA gene (rrs) sequencing. The same isolates were examined for their hemolytic activity on sheep blood agar, DNase activity, and coagulase activity and with two rapid identification kit...

AIM This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt. MATERIALS AND METHODS A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotyp...

Candida coagulase production was assessed by the classical tube test. All Candida krusei strains were coagulase negative, but most C. albicans and C. tropicalis strains can produce coagulase. Some strains agglutinated the Pastorex Staph-Plus reagent, probably because of antigen similarities to coagulase produced by Staphylococcus aureus that may cause mistakes in clinical laboratories.

Abstract Background Staphylococcus aureus infections are remarkable due to their high morbimortality. In addition, antibiotic resistance of these bacteria is a cause concern since it worsens the patient prognosis and associated higher morbidity, mortality cost treatment. The aim this study was assess clinical performance assay VIASURE Methicillin resistant Real Time Detection Kit in epidemiolog...