background and objectives: to investigate coagulase gene polymorphisms of mrsa and mssa isolates from shiraz teaching hospitals from 2011 to 2012. materials and methods: a total of 302 isolates of staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. the isolates were subj...

BACKGROUND Identification of Staphylococci to species level in veterinary microbiology is important to inform therapeutic intervention and management. We report on the efficacy of three routinely used commercial phenotypic methods for staphylococcal species identification, namely API Staph 32 (bioMérieux), RapID (Remel) and Staph-Zym (Rosco Diagnostica) compared to genotyping as a reference met...

conclusions the cefoxitin disk diffusion method showed the best results when compared to oxacillin disk, similar to results from detecting the meca gene in pcr as a golden test. studies on mrsa that carry the luk-pv gene should continue to provide significant insight into the prevalence and epidemiology of these important resistant pathogens. also, the rate of pvl gene-producing isolates showed...

A total of 288 staphylococcal specimens isolated from different clinical specimens were selected for the evaluation of tests used to detect Staphylococcus aureus. The coagulase (coa) gene PCR was preformed, which confirmed 288 specimens as S. aureus and 51 specimens as coagulase negative staphylococci (CONS). All the specimens were subjected to slide coagulase test, Slidex Staph plus test and t...

One hundred and ninety eight samples of human and animal sources from different areas in El-Sharkia Governorate were used in this study to isolate Staphylococcus species and to determine the presence of coagulase ( coa) gene,a virulence gene detected in S. aureus (CoPS), by PCR. Primary isolation on mannitol salt agar, $-haemolysis, tube coagulase test (TCT) and other biochemical characterizati...

Exploration of pathogenic potential Coagulase-Negative Staphylococci (CoNS), in human as well animals,gained the importance during past two decades. Emergence Methicillin-Resistant Coagulase-NegativeStaphylococci (MRCoNS) bovine milk poses to be a major public health concern. Presence mecA gene isone most common reasons reported for methicillin resistant bacteria. Since scientific data concerni...

Staphylococcus aureus (including Methicillin-resistant S. aureus) and coagulase negative Staphylococcus spp (including methicillin-resistant coagulase negative) permanently or transiently colonize humans and act as sources of complicated skin and soft tissue infections, bacteraemia and infective endocarditis. They also cause chronic infection of indwelling medical devices. Treatment of these in...

The objective of this study was to evaluate the prevalence of coagulase-negative staphylococci in healthy dogs and to determine whether methicillin-resistant staphylococci expressed the mecA gene. Nasal and rectal swab samples were taken from 50 clinically healthy dogs. The prevalence of coagulase-negative staphylococci was evaluated according to phenotypic properties. The agar diffusion method...

Background: Recent studies have shown an increasing incidence of antibiotic resistance in dacryocystitis. Management diseases may include determining microbial agents and choosing appropriate antibiotics for treatment. Objectives: This study aimed to present the best treatments To this end, specimens' microbiology susceptibility were examined patients with dacryocystitis laboratory Kashan Unive...