We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...

background: the aim was to compare three different methods (direct examination, culture and pcr meth­ods) for the diagnosis of acanthamoeba keratitis (ak) in corneal scrapes. methods: twenty eight corneal scrapes and contact lenses were collected from keratitis patients and re­ferred to the de­partment of medical parasitology and mycology, school of public health, tehran univer­sity of medical ...

we investigated the use of dna amplification by polymerase chain reaction (per) for detection of mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with pcr. two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared for dna...

abstract background brucellosis is endemic in iran, and is seen in all age groups, including children. its diagnosis in childhood needs a high index of suspicion. the diagnostic methods currently in use need evaluation for analytical performance. objective the present study tries to evaluate the hematological indices, serologic markers of inflammation and symptoms in patients suspected of bruce...

streptococcus agalactiae , s. dysgalactiae and s. uberis are gram-positive bacterial pathogens that affect cows in dairy herds. these are considered as the major causes of economic losses of dairy producers without a control program. to reveal the role of these agents in inducing subclinical mastitis in dairy cattle in ahvaz, 100 cmt-positive milk samples and 20 cmt-negative milk samples were c...

  A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...

  Background :Polymerase chain reaction (PCR) assay has widely used for the detection of tuberculosis (TB). This study tried to compare in-house PCR with some well-known commercial PCR kits for detection of TB agent.   Methods : Clinical samples obtained from 620 TB suspected patients were analyzed for the diagnosis of Mycobacterium tuberculosis complex (MTC) by in-house PCR. All samples were o...