Plant products provide a vast source of therapeutics, but not without toxicity. This study evaluated the in vitro cytotoxic and proliferative activity selected plant extracts on human peripheral blood mononuclear cells (PBMCs). The PBMCs from healthy donors were exposed to varying concentrations (25 µg/ml, 50 100 µg/ml 200 µg/ml) aqueous young leaves Mangifera indica (MI) stem barks Commiphora ...

This study aims to design and characterize the layer-by-layer assembly of core-corona nanoarchitecture for novel surface-modified solid lipid nanoparticles. Oppositely charged ?-cyclodextrin polymers were used build corona structure onto core, particle size, polydispersity index, zeta potential SLN with polymer layers evaluated. Morphology was identified using TEM. The effect coating on drug re...

Introduction. In our previous study, it has been confirmed that formaldehyde (FA) not only inhibits the proliferative activity, but also causes DNA-protein crosslinks (DPCs) formation in bone marrow mesenchymal stem cells (BMSCs). The purpose of this study was to detect protective effect astragalus polysaccharide (APS) against cytotoxicity and genotoxicity BMSCs exposed FA, explore potential mo...

Scorpion venom toxicity is of major concern due to its influence on human activities and public health. We investigated the in-vitro process of cell death caused by two Iranian scorpions Odontobuthus doriae and Bothutus salceyi venom on human cell lines. The aim of this study was to provide further information about triggering cell death and suggestion of methods for the elimination of unwanted...

Fluorometric assays of cell viability and cytotoxicity are easy to perform with the use of a fluorescence microscope, fluorometer, fluorescence microplate reader or flow cytometer,1 and they offer many advantages over traditional colorimetric and radioactivity-based assays. This section describes our numerous reagents for conducting viability and cytotoxicity assays in a wide variety of cells, ...

This paper proposes a new cytotoxicity assay in a microfluidic device with microwells and a distributive microfluidic channel network for the formation of cancer cell spheroids. The assay can generate rapid and uniform cell clusters in microwells and test in situ cytotoxicity of anticancer drugs including sequential drug treatments, long term culture of spheroids and cell viability assays. Inle...

Quercetin, a ubiquitous flavonol, represents a promising leading drug for development of new chemotherapeutic agents. However, its limited cytotoxicity to cancer cells hampers its clinical use. In order to obtain novel quercetin derivatives with superior cytotoxicity, seven alkylated quercetin derivatives were synthesized. Solubility of these derivatives was determined by turbidimetry. Cytotoxi...

objective: resin cements, regardless of their biocompatibility, have been widely used in restorative dentistry during the recent years. these cements contain hydroxy ethyl methacrylate (hema) molecules which are claimed to penetrate into dentinal tubules and may affect dental pulp. since tooth preparation for metal ceramic restorations involves a large surface of the tooth, cytotoxicity of thes...

in this study cellular phosphatase activity, referred to as enzyme developed color (edc) assay, was used for an in vitro assessment of cell proliferation and cytotoxicity. optimum conditions were established and found to be 10 x103 cells / well at 37°c and 60 min incubation (for developing color). under the same conditions a direct correlation between optical density (00) and cell number was ob...

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...