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Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a di...

We have isolated a novel protein kinase cDNA, PfPK6, by differential display RT-PCR (DDRT-PCR) of mRNA obtained from different asexual erythrocytic stages of Plasmodium falciparum, which shows sequence similarity to both cyclin-dependent kinase (CDK) and mitogen-activated protein kinase (MAPK) family members. The 915 bp open reading frame (ORF) is interrupted by seven introns and encodes a 305-...

Establishment of a plant-pathogen interaction involves differential gene expression in both organisms. In order to isolate Botrytis cinerea genes whose expression is induced during its interaction with tomato, a comparative analysis of the expression pattern of the fungus in planta with its expression pattern during in vitro culture was performed by differential display of mRNA (DDRT-PCR). Disc...

In an effort to characterize molecular events contributing to lineage commitment and terminal differentiation of stem/progenitor cells, we have used differential display reverse transcription polymerase chain reaction (DDRT-PCR) and cell lines blocked at two distinct stages of differentiation. The cell lines used were EML, which is representative of normal multipotential primitive progenitors (...

Academic dissertation To be presented for public criticism, with permission of the Abbreviations ABA abscisic acid HRGP hydroxyproline-rich glycopro-tein AOX alternative oxidase JA jasmonic acid APX ascorbate peroxidase MJ methyl jasmonate ARE antioxidant responsive element MPT1 mitochondrial phosphate translocator1 CAT catalase MRE metal responsive element Cat1AS CAT1 antisense mRNA messenger ...

Evaluation of in vivo gene expression in specific types of cells is difficult because animal tissues are complex mixtures of many cell types, and RNA extracted from homogenized fresh whole tissue is derived from all cell types present. In an attempt to improve specificity, RNA has been isolated from specific cells microdissected from fixed, paraffin-embedded tissue sections (2,6), but the yield...