background: ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. in the current study, the purpose was to evaluate the effects of ammuniom chloride (ac) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of bcl-2. methods: in vitro maturation (ivm) was performed in t...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO2 in...

Objective The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods In this experimental study, th...

BACKGROUND Human embryonic stem cells (hESCs) may provide an invaluable resource for regenerative medicine. To move hESCs towards the clinic it is important that cells with therapeutic potential be reproducibly generated under completely defined conditions. METHODOLOGY/PRINCIPAL FINDINGS Here we report a four-step scalable process that is readily transferable to a Good Manufacture Practice (G...

The purpose of the current study was to investigate the maturation of form and motion perception, specifically the component visual abilities involved in the identification of motion-defined form, in children ranging in age from 3 to 12 years. Experiment 1 compared the maturation of motion-defined and texture-defined shape identification. Minimum speed thresholds on the motion-defined shape tas...