Capilla, Encarnación, Mònica Dı́az, Joaquim Gutiérrez, and Josep V. Planas. Physiological regulation of the expression of a GLUT4 homolog in fish skeletal muscle. Am J Physiol Endocrinol Metab 283: E44–E49, 2002. First published March 12, 2002; 10.1152/ajpendo.00065.2002.—We have recently cloned a glucose transporter from brown trout muscle (btGLUT) with high sequence homology to mammalian GLUT4...

Background and Purpose—Inadvertent contrast agent injection in the left cubital artery may lead to decisively altered perfusion parameters in stroke CT. These effects have not previously been described. Summary of Case—A 77-year-old woman with a suspected stroke underwent subsequent stroke CT imaging. No signs of intracranial hemorrhage or acute cerebral ischemia were noted on nonenhanced CT. Q...

Optimizing design of vectors is critical to effective gene therapy. In targeted gene correction (TGC), cleavage of chromosomal DNA near a mutation stimulates homology-directed repair of a target gene using a donor provided in trans. We have systematically addressed epigenetic parameters of donor design, using a flow-based assay to quantify correction frequencies and expression levels of a green...

Gap junctions formed between transfected cells expressing connexin (Cx) 40 and Cx43 (Cx43-RIN, Cx40-HeLa, and Cx43-HeLa) revealed a relationship, gj5f(Vj), at steady state, that is typified by a nonsymmetrical behavior similar to that previously reported for other heterotypic channels (gap junction conductance [gj]; transjunctional voltage [Vj]). The unitary conductance of the channels was sens...

Cancer sequencing studies are increasingly comprehensive and well powered, returning long lists of somatic mutations that can be difficult to sort and interpret. Diligent analysis and quality control can require multiple computational tools of distinct utility and producing disparate output, creating additional challenges for the investigator. The Cancer-Related Analysis of Variants Toolkit (CR...

We investigated hydrothermal liquefaction of Nannochloropsis sp. at different temperatures (250e400 C), times (10e90 min), water densities (0.3e0.5 g/mL), and biomass loadings (5 e35 wt %). Liquefaction produced a biocrude with light and heavy fractions, along with gaseous, aqueous, and solid by-product fractions. The gravimetric yields of the product fractions from experiments at 250 C summed ...

aCGH: array comparative genomic hybridization FFPE: formalin-fixed paraffin-embedded MCC: Merkel cell carcinoma MCpV: Merkel cell polyomavirus PET/CT: positron emission tomography/ computed tomography SLNB: sentinel lymph node biopsy INTRODUCTION In the United States, the incidence and mortality rates for Merkel cell carcinoma (MCC) have more than tripled in the last 2 decades. The main risk fa...

Macrophages and neutrophils play important roles during the innate immune response, phagocytosing invading microbes and delivering antimicrobial compounds to the site of injury. Functional analyses of the cellular innate immune response in zebrafish infection/ inflammation models have been aided by transgenic lines with fluorophore-marked neutrophils. However, it has not been possible to study ...