In this study, Glucoamylase production by Aspergillus Niger was investigated under solid state conditions with low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Different additions of (CF) were tested and WB+ 10% CF showe...

aloe vera is a plant of the liliaceae family with a brilliant history in disease treatment. in addition to the pharmaceutical, cosmetic, and health care industries, this plant is used in food industry due to having various nutrients such as amino acids and various vitamins or anti-bacterial compounds. the purpose of this study was to investigate the effects of variables such as temperature, tim...

An RNA enzyme has been developed that catalyzes the joining of oligonucleotide substrates to form additional copies of itself, undergoing self-replication with exponential growth. The enzyme also can cross-replicate with a partner enzyme, resulting in their mutual exponential growth and enabling self-sustained Darwinian evolution. The opportunity for inventive evolution within this synthetic ge...

The Front Cover shows that a medium-chain alcohol dehydrogenase modified in the loop is efficiently catalyzing N-Boc-3-pyrrolidone to produce (S)-N-Boc-3-pyrrolidinol. In their Research Article, H. Wang and co-workers proposed loop-loop interaction region acts like “bridges” between loops could be used adjust conformational dynamics of enzyme, thus modifying its catalytic efficiency. Combined w...

Modulation of enzyme activity is a powerful means of probing cellular function and can be exploited for diverse applications. Here, we explore a method of enzyme activation where covalent tethering of a small molecule to an enzyme can increase catalytic activity (kcat/KM) up to 35-fold. Using a bacterial glycoside hydrolase, BtGH84, we demonstrate how small molecule "fragments", identified as a...