1,3,5(10),16-Estratetraen-3-01 (estratetraenol) that has previotisly been found in the urine of women in late pregnancy and proved to be biosynthesized in human placenta from 4,16-androstadien-3-one, a hepatic metabolite of testosterone, was converted under aerobic conditions by female rat liver microsomes into estratetraenol 16a,l7a-epoxide, estratetraenol 16P,17P-epoxide, 16,17-epiestriol, an...

leishmaniasis has a wide spectrum of signs and symptoms due to infection to numbers of leishmania species and makes enormous mortality and morbidity. there are clues of antileishmanial effects of prenylated coumarins. apiaceae family is one of the most important sources of coumarins. air-dried aerial parts of ferulago angulata and fruits of prangos asperula were extracted with n -hexane, using ...

The tumorigenicity of chrysene, (+)and (-)-frans-1,2-dihydroxy-1,2-dihydrochrysene (chrysene 1,2-dihydrodiol), and the ( + )and (—)-enantiomers of the diastereomeric bay-region chrysene 1,2-diol-3,4-epoxides was assessed in two tumor models. A single topical application of 0.4 or 1.2 /¿mol of the chrysene derivatives on mouse skin followed by 25 weeks of promotion with 12-O-tetradecanoylphor...

We have utilized a DNA clone complementary to epoxide hydrolase mRNA as a probe to examine the level of the mRNA in persistent hepatocyte nodules and hepatomas induced by the Solt-Farber chemical carcinogenesis procedure. Epoxide hydrolase mRNA is increased 14-fold in nodules as compared to the level in normal liver. When rats with liver nodules were administered phenobarbital, an inducer of ep...

Epoxide carboxylase from Xanthobacter strain Py2 catalyzes the reductant- and NAD+-dependent carboxylation of aliphatic epoxides to beta-keto acids. Epoxide carboxylase from Xanthobacter strain Py2 has been resolved from cell extracts by anion-exchange chromatography into three protein components, designated I, II, and III, that are obligately required for functional reconstitution of epoxide c...

The epoxide hydrolase gene from Agrobacterium radiobacter AD1, a bacterium that is able to grow on epichlorohydrin as the sole carbon source, was cloned by means of the polymerase chain reaction with two degenerate primers based on the N-terminal and C-terminal sequences of the enzyme. The epoxide hydrolase gene coded for a protein of 294 amino acids with a molecular mass of 34 kDa. An identica...

A growing body of evidence implicates epoxide metabolites of mutagenic and carcinogenic polycyclic hydrocarbons as either the only species, or one of the contributing species responsible for these adverse effects. Selective induction of epoxide hydratase(s) catalyzing the transformation of epoxides to electrophilically unreactive dihydrodiols, under conditions not leading to increases in monoox...

Styrene has been found to be toxic to the respiratory system, and the toxicity of styrene is metabolism-dependent. CYP2E1 is suggested to be one of the cytochrome P450 enzymes responsible for the bioactivation of styrene. Our work focused on the roles of CYP2E1 and epoxide, a metabolite of styrene epoxidation, in the cytotoxicity of styrene. Styrene was found to be more toxic to h2E1 cells than...

ing operations involvig the use of a bisphenol-A diglycidyl ether tye of epoxy resin. ln a faciity producig miltaiy aircraft, the use of an epoxy prier did not result in detectable epoxide levels, and the authors surmised that the epoxy-amine curig reaction had probably . consumed most of the epoxide group. Epoxide levels of 2-12 J.Eq/m3 epode functional group were recorded durig the painting o...