Phospholipid metabolism in the lung was studied because the lung was the first organ at which the ingested lipids arrived. It was revealed in this study that the lung contained phosphatidyl cho-line, ethanolamine, phosphoinositol, sphingomyelin and lysolecithin, that the amount of phosphatidyl serine were very small in the lung, and that a phosphatidic acid was discovered in the lung after the ...

A 51 year old man with Waldenström's macroglobulinaemia presented with a malabsorptive syndrome related to extensive small bowel lymphangiectasia caused by immunoglobulin accumulation. The patient's plasma had strong lupus anticoagulant activity and the IgM lambda paraprotein displayed specificity for the negatively charged phospholipids phosphatidyl serine and phosphatidyl inositol, as well as...

Phospholipids of amniotic fluid were extracted, separated by thin-layer chromatography, detected with acidified ammonium sulfate, and charred; the phosphatidyl choline and sphingomyelin fractions were quantitated by transmission densitometry.The densities were recorded as integrator trace units.We used commercially available precoated plates and a solvent system to eliminate the interference of...

The C2 domain of protein kinase Calpha (PKCalpha) corresponds to the regulatory sequence motif, found in a large variety of membrane trafficking and signal transduction proteins, that mediates the recruitment of proteins by phospholipid membranes. In the PKCalpha isoenzyme, the Ca2+-dependent binding to membranes is highly specific to 1,2-sn-phosphatidyl-l-serine. Intrinsic Ca2+ binding tends t...

Several receptors are implicated in apoptotic cell (AC) uptake by phagocytic cells; however, their relative dominance in mammalian systems remains to be established. New studies shed light on the role of the phosphatidyl serine (PS) receptor (PSR). Ligation of PSR by PS on AC surfaces is considered essential for signaling uptake of ACs that are tethered to phagocytes via other receptors.

The glycoprotein bovine lactadherin (formerly known as PAS-6/7) comprises two EGF-like domains and two C-like domains found in blood clotting factors V and VIII. Bovine lactadherin binds to alpha(v)beta(5) integrin in an RGD-dependent manner and also to phospholipids, especially phosphatidyl serine. To define and characterize these bindings the interactions between lactadherin and different mam...