* The trichloroacetic acid extracts were first acidified with sulfuric acid3 and kept overnight at 38” for decarboxylation of acetoacetate. The fluid was then neutralized with sodium hydroxide and phosphate, and the acetone distilled by the procedure of Folin.4 Acetone was determined calorimetrically by the method of Behre.6 Analogous results were obtained with the determination procedure of Le...

It would be desirable to establish and standardize methods that can measure the total antioxidant capacity level directly from vegetable extracts containing phenolics. Antioxidant capacity assays may be broadly classified as electron transfer (ET)- and hydrogen atom transfer (HAT)-based assays. The majority of HAT assays are kinetics-based, and involve a competitive reaction scheme in which ant...

The first review of the literature on creatine and creatinine after the advent of the Folin colorimetric method was presented by Professor Mendel22 before a joint meeting of the Section on Physiology and Experimental Medicine of the American Association for the Advancement of Science and the biochemical, physiological and bacteriological societies in Baltimore in 1908. Although only four years ...

ITIS WELL RECOGNIZED that the Folin phenol method (1) for the determination of protein is not specific. This method was in fact originally standardized with protein or tyrosine, and the Folin phenol reagent was shown to react with numerous mono-, di-, and trihydroxyphenols in addition to other substances (s). Although most of these substances are not present in sufficient amounts to interfere w...

This procedure for determining serum proline in patients with hyperprolinemia involves protein precipitation (Folin-Wu method), color development with isatin, extraction of the color with methylene chloride and measurement of its absorbance at 600 nm. The specificity and analytical recovery show the method to be suitable for this use.