DNA damage checkpoint activation can be subdivided in two steps: initial activation and signal amplification. The events distinguishing these two phases and their genetic determinants remain obscure. TopBP1, a mediator protein containing multiple BRCT domains, binds to and activates the ATR/ATRIP complex through its ATR-Activation Domain (AAD). We show that Schizosaccharomyces pombe Rad4(TopBP1...

A series of 1,2,4 triazole derivatives (H7-12) have been synthesized by reacting an excess hydrazine hydrate with carbothioamide (H1-6). The final compounds (HB1-HB6) were the mefenamic acid using DCC as a coupling agent. chemical structures confirmed FT-IR, 1 H, and 13 C-NMR spectra, some physicochemical properties determined. cytotoxicity different was evaluated MTT assay against two human ep...

N-Butylidenephthalide (BP), which is extracted from a traditional Chinese medicine, Radix Angelica Sinensis (danggui), displays antitumor activity against various cancer cell lines. The purpose of this study was to investigate the cytotoxic and radiosensitizing effect of BP and the underlying mechanism of action in human breast cancer cells. BP induces apoptosis in breast cancer cells, which wa...

We previously generated a monoclonal antibody (mAb), G2, by immunizing mice with Residues 174-247 of the chicken prion protein (ChPrP(C) ). In this study, we found that G2 possessed an extremely unusual characteristic for a mAb; in particular, it could react with at least three proteins other than ChPrP(C) , the original antigenic protein. We immunoscreened a complementary DNA library from chic...

In this research, we developed a novel chimeric HTNV-IL-2-G2 DNA vaccine plasmid by genetically linking IL-2 gene to the G2 segment DNA and tested whether it could be a candidate vaccine. Chimeric gene was first expressed in eukaryotic expression system pcDNA3.1 (+). The HTNV-IL2-G2 expressed a 72 kDa fusion protein in COS-7 cells. Meanwhile, the fusion protein kept the activity of its parental...

Abstract Aurora B kinase is aberrantly overexpressed in various tumors and shown to be a promising target for anti-cancer therapy. In human oral squamous cell carcinoma (OSCC), the high protein level of required maintaining malignant phenotypes, including vitro growth, colony formation, vivo tumor development. By molecular modeling screening 74 commercially available natural products, we identi...

CDC25B phosphatase plays a key role in controlling G2-M progression by dephosphorylating two inhibitory residues of CDC2 and also has been suggested to have an oncogenic property. In this study, we investigated the effect of CDC25B overexpression on radiation-induced G2-M arrest and radiation sensitivity in esophageal cancer cells. TE8-CDC25B, in which CDC25B was overexpressed under an inducibl...

Fragile X syndrome, the most common form of inherited mental retardation, is caused by the loss of the fragile X mental retardation protein (FMRP). FMRP is a ubiquitously expressed, multi-domain RNA-binding protein, but its in vivo function remains poorly understood. Recent studies have shown that FMRP participates in cell cycle control during development. Here, we used Drosophila mutants to te...

Purpose: To synthesize and characterize some new gabapentin Schiff base derivatives, to assess their antibacterial, antioxidant antiepileptic activities.Methods: Four derivatives of gabapentin, termed G1, G2, G3 G4, were synthesized by condensation with benzoin, vanillin, acetophenone, benzophenone, respectively. Their chemical identities established FTIR, 1 H NMR 13C techniques. The compounds ...

Objective: In a previous work carried on in our laboratory, positive correlation were recorded between different levels of vitamin D and the antioxidant status Iraqi patients with rheumatoid arthritis, meanwhile some peroxidases family have been known to act as antioxidant. Therefore order look for molecular level this action D, impact activity arthritis was investigated. Method: The study incl...