The locus of enterocyte effacement (LEE) pathogenicity island of enterohemorrhagic Escherichia coli (EHEC) O157:H7 possesses the same genes in identical order and orientation as the LEE of enteropathogenic E. coli (EPEC) O127:H6 but is unable to form attaching and effacing (A/E) lesions or to secrete Esp proteins when it is cloned in an E. coli K-12 background. The A/E phenotype could not be re...

Here, we report the diversity of amantadine-resistant mutants among avian influenza A viruses with pandemic potential (H5, H6, H7, and H9 hemagglutinin subtypes). Drug-resistant variants were not detected among 1979--83 isolates, whereas 31.1% of H5 and 10.6% of H9 strains from Southeast Asia isolated in 2000-04 carried mutations in M2 protein. In North America, resistant variants occurred amon...

To identify mannosyl (Man)-containing intermediates of the human glycoinositol phospholipid (GPI) anchor pathway and examine their expression in paroxysmal nocturnal hemoglobinuria (PNH), mannolipid products deriving from in vitro guanosine diphosphate [3H]Man labeling of HeLa cell microsomes were characterized. The defined GPI species were correlated with products deriving from in vivo [3H]Man...

derivative3–6 (1) have been reported to have a greater tendency to yield polynuclear complexes. This communication presents the X-ray structure of the Ni–Mn–Ni complex (2) prepared using ligand 1 (Fig. 1). The title complex was prepared in two steps. Step 1: After the ligand (0.01 mol, 2.98 g) was dissolved in hot EtOH (50 mL), a solution of NiCl2·6H2O (0.01 mol, 2.37 g) in hot water (30 mL) an...

We have determined the structure of bovine rhodopsin at 2.65 A resolution using untwinned native crystals in the space group P3(1), by molecular replacement from the 2.8 A model (1F88) solved in space group P4(1). The new structure reveals mechanistically important details unresolved previously, which are considered in the membrane context by docking the structure into a cryo-electron microscop...

Первые правовые нормы, предусматривающие уголовную ответственность за сговоры, ограничивающие конкуренцию, появились в Российском царстве еще середине XVII в. За более чем три столетия, прошедших с этого времени, законодатель не раз достаточно кардинально менял свое отношение к антиконкурентным сговорам — изменялись виды запрещенных соглашений, объекты таких посягат...

A working structural model of penicillin-binding protein 1B (PBP 1B) from Escherichia coli derived from previous data consists of a highly charged aminoterminal cytoplasmic tail, a 23-amino-acid hydrophobic transmembrane anchor, and a 758-amino-acid periplasmic domain. Using an engineered thrombin cleavage site, we have investigated the solubility properties of the periplasmic domain of PBP 1B....